| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TMEM63A |
| Uniprot No | O94886 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-807 aa |
| 活性数据 | MMDSPFLELWQSKAVSIREQLGLGDRPNDSYCYNSAKNSTVLQGVTFGGIPTVLLIDVSCFLFLILVFSIIRRRFWDYGRIALVSEADSESRFQRLSSTSSSGQQDFENELGCCPWLTAIFRLHDDQILEWCGEDAIHYLSFQRHIIFLLVVVSFLSLCVILPVNLSGDLLDKDPYSFGRTTIANLQTDNDLLWLHTIFAVIYLFLTVGFMRHHTQSIKYKEENLVRRTLFITGLPRDARKETVESHFRDAYPTCEVVDVQLCYNVAKLIYLCKEKKKTEKSLTYYTNLQVKTGQRTLINPKPCGQFCCCEVLGCEWEDAISYYTRMKDRLLERITEEERHVQDQPLGMAFVTFQEKSMATYILKDFNACKCQSLQCKGEPQPSSHSRELYTSKWTVTFAADPEDICWKNLSIQGLRWWLQWLGINFTLFLGLFFLTTPSIILSTMDKFNVTKPIHALNNPIISQFFPTLLLWSFSALLPSIVYYSTLLESHWTKSGENQIMMTKVYIFLIFMVLILPSLGLTSLDFFFRWLFDKTSSEASIRLECVFLPDQGAFFVNYVIASAFIGNGMELLRLPGLILYTFRMIMAKTAADRRNVKQNQAFQYEFGAMYAWMLCVFTVIVAYSITCPIIAPFGLIYILLKHMVDRHNLYFVYLPAKLEKGIHFAAVNQALAAPILCLFWLYFFSFLRLGMKAPATLFTFLVLLLTILVCLAHTCFGCFKHLSPLNYKTEEPASDKGSEAEAHMPPPFTPYVPRILNGLASERTALSPQQQQQQTYGAIHNISGTIPGQCLAQSATGSVAAAPQEA |
| 分子量 | 118.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TMEM63A蛋白的参考文献示例(内容基于模拟，建议通过学术数据库核实准确性)：
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1. **标题**: *Structural insights into the mechanosensitive ion channel TMEM63A in lipid bilayers*
**作者**: Zhang Y, et al.
**摘要**: 本研究利用冷冻电镜技术解析了重组人TMEM63A蛋白在脂质双分子层中的三维结构，揭示其机械激活机制。研究显示TMEM63A形成同源三聚体，胞外结构域可能参与感知膜张力变化。
2. **标题**: *Functional characterization of recombinant human TMEM63A in HEK293 cells*
**作者**: Li X, Chen Q.
**摘要**: 通过在HEK293细胞中过表达重组TMEM63A，发现其具有渗透压敏感性离子通道功能。钙成像实验表明，TMEM63A可被低渗刺激激活，提示其在细胞体积调节中的作用。
3. **标题**: *TMEM63A mutations linked to neonatal hypomyelination disrupt protein stability*
**作者**: Wang R, et al.
**摘要**: 分析了与婴儿脑白质病变相关的TMEM63A突变体。通过体外重组蛋白表达发现，部分突变导致蛋白错误折叠和内质网滞留，影响其离子通道功能。
4. **标题**: *TMEM63A interacts with cytoskeletal proteins via its C-terminal domain*
**作者**: González-Morales M, et al.
**摘要**: 利用重组人TMEM63A蛋白的C端截断体进行免疫共沉淀实验，发现其与β-actin和微管蛋白存在相互作用，提示机械传导可能与细胞骨架动态相关。
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建议通过 **PubMed、Web of Science** 或 **Google Scholar** 搜索最新文献，使用关键词“TMEM63A recombinant”“TMEM63A ion channel”或结合具体研究主题进行扩展检索。
TMEM63A is a member of the transmembrane protein 63 (TMEM63) family, primarily known for its role as a mechanosensitive ion channel. It is widely expressed in mammalian tissues, particularly in the central nervous system, kidneys, and lungs. Structurally, TMEM63A contains six transmembrane domains and is postulated to form pores that facilitate ion transport in response to mechanical stimuli, such as changes in membrane tension or osmotic pressure. Studies suggest its involvement in osmosensing and maintaining cellular homeostasis by regulating ion flux across membranes.
Recombinant human TMEM63A protein is generated using heterologous expression systems (e.g., HEK293 cells) to study its biophysical properties, channel activity, and interaction partners. Research has linked TMEM63A mutations to severe neurodevelopmental disorders, including infantile-onset transient hypomyelination and progressive encephalopathy, highlighting its critical role in neuronal function. Its recombinant form enables detailed analysis of pathogenic variants and screening for therapeutic agents.
Beyond neurology, TMEM63A is implicated in renal function and pulmonary surfactant secretion, though its mechanisms remain under investigation. The protein’s mechanosensitivity also makes it a candidate for exploring cellular responses to physical forces in health and disease. Ongoing studies aim to clarify its physiological roles and potential as a biomarker or drug target.
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