| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TMEM50B |
| Uniprot No | P56557 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-158 aa |
| 活性数据 | MAGFLDNFRWPECECIDWSERRNAVASVVAGILFFTGWWIMIDAAVVYPKPEQLNHAFHTCGVFSTLAFFMINAVSNAQVRGDSYESGCLGRTGARVWLFIGFMLMFGSLIASMWILFGAYVTQNTDVYPGLAVFFQNALIFFSTLIYKFGRTEELWT |
| 分子量 | 44.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TMEM50B蛋白的3篇参考文献示例(注：以下文献为模拟虚构，实际文献需通过学术数据库查询)：
1. **文献名称**：*TMEM50B promotes cell proliferation and invasion in hepatocellular carcinoma via EGFR signaling*
**作者**：Chen, L., et al.
**摘要**：研究通过重组人TMEM50B蛋白过表达实验，发现其通过激活EGFR通路增强肝癌细胞的增殖和侵袭能力，提示TMEM50B可能作为肝癌治疗的潜在靶点。
2. **文献名称**：*Expression and purification of recombinant human TMEM50B in Escherichia coli for structural analysis*
**作者**：Wang, Y., et al.
**摘要**：报道了一种高效的原核表达系统用于重组TMEM50B蛋白的制备，通过优化诱导条件获得可溶性蛋白，并利用质谱和圆二色谱验证其结构完整性。
3. **文献名称**：*TMEM50B interacts with VAPA to regulate ER membrane morphology and autophagy*
**作者**：Zhang, H., et al.
**摘要**：利用重组TMEM50B蛋白进行免疫共沉淀实验，发现其与内质网膜蛋白VAPA相互作用，共同调控内质网形态和细胞自噬过程。
建议通过PubMed、Google Scholar等平台以关键词“TMEM50B recombinant”或“TMEM50B protein function”检索真实文献。如需具体论文协助，可提供进一步信息。
**Background of Recombinant Human TMEM50B Protein**
Transmembrane protein 50B (TMEM50B), also known as C21orf4 or GSAP (γ-secretase activating protein), is a conserved eukaryotic protein located on human chromosome 21q22.11. It encodes a multi-pass transmembrane protein predominantly localized in the endoplasmic reticulum (ER) and nuclear envelope. TMEM50B is implicated in membrane-associated processes, including vesicular trafficking, protein maturation, and cellular homeostasis.
Structurally, TMEM50B contains four predicted transmembrane domains and a cytosolic C-terminal region, suggesting roles in membrane scaffolding or protein-protein interactions. Notably, its interaction with γ-secretase links it to amyloid-beta (Aβ) production in Alzheimer’s disease, as GSAP regulates γ-secretase activity by stabilizing substrate access. TMEM50B is also associated with cancer progression, immune responses, and other neurological disorders.
Recombinant human TMEM50B protein is typically produced using mammalian or bacterial expression systems, enabling functional studies of its biochemical properties, interactome, and disease mechanisms. Its purification often involves affinity tags (e.g., His-tag) followed by chromatography. Research applications include elucidating its role in Aβ pathology, drug target validation for neurodegenerative diseases, and exploring its regulatory effects on membrane dynamics. Further studies aim to clarify its broader physiological and pathological significance.
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