| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TMEM41B |
| Uniprot No | Q5BJD5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-192 aa |
| 活性数据 | MAKGRVAERSQLGAHHTTPVGDGAAGTRGLAAPGSRDHQKEKSWVEAGSARMSLLILVSIFLSAAFVMFLVYKNFPQLSEEERVNMKVPRDMDDAKALGKVLSKYKDTFYVQVLVAYFATYIFYQLANICYSRLYISQYTLRVSLSLSTSLISCLFVFWTWCLFLLYAFLFSWETSCIQIPNRESSKMVTAG |
| 分子量 | 48.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与TMEM41B蛋白相关的文献摘要概览：
1. **文献名称**: "TMEM41B is a novel regulator of autophagy and lipid mobilization"
**作者**: Morishita H, et al.
**摘要**: 该研究首次揭示TMEM41B通过调节内质网膜的形态变化，参与自噬体形成和细胞内脂质代谢，基因敲除导致自噬缺陷和脂滴积累。
2. **文献名称**: "A global genetic interaction network maps a wiring diagram of cellular function"
**作者**: Costanzo M, et al.
**摘要**: 通过全基因组筛选发现，TMEM41B与VMP1等膜蛋白共同调控细胞器膜重组，影响病毒复制(如黄病毒)和脂类运输，提示其在跨膜通路中的关键作用。
3. **文献名称**: "TMEM41B acts as a host factor for SARS-CoV-2 infection"
**作者**: Schneider WM, et al.
**摘要**: 功能性基因组学研究表明，TMEM41B通过调控胆固醇稳态和内膜系统，促进新冠病毒复制，其缺失显著降低病毒在肺细胞中的增殖能力。
*注：如需具体文献，可基于PMID或DOI在PubMed等平台检索原文。*
TMEM41B (Transmembrane Protein 41B) is a conserved endoplasmic reticulum (ER)-resident protein implicated in diverse cellular processes, including autophagy, lipid metabolism, and organelle membrane remodeling. First characterized in 2018. it spans the ER membrane multiple times, though its exact topology remains under investigation. TMEM41B facilitates autophagosome formation by promoting lipid mobilization and membrane curvature, likely through its putative lipid scramblase activity. It also interacts with vacuole membrane protein 1 (VMP1), another ER-phagy-related protein, to regulate ER homeostasis.
Notably, TMEM41B has been identified as a critical host factor for flavivirus replication (e.g., Zika, dengue), suggesting its role in viral membrane biogenesis. Genetic studies link TMEM41B variants to metabolic disorders and neurodegenerative diseases, with mouse knockout models showing lipid accumulation, autophagy defects, and early mortality.
Recombinant human TMEM41B protein, typically expressed in mammalian systems like HEK293 cells, retains post-translational modifications for functional studies. It serves as a tool to investigate lipid-protein interactions, screen antiviral compounds, and characterize mutations. Current challenges include resolving its 3D structure and elucidating molecular mechanisms of its lipid-handling functions. Its dual role in cellular homeostasis and viral pathogenesis makes TMEM41B a compelling target for therapeutic development.
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