| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TMEM41A |
| Uniprot No | Q96HV5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-264 aa |
| 活性数据 | MRPLLGLLLVFAGCTFALYLLSTRLPRGRRLGSTEEAGGRSLWFPSDLAELRELSEVLREYRKEHQAYVFLLFCGAYLYKQGFAIPGSSFLNVLAGALFGPWLGLLLCCVLTSVGATCCYLLSSIFGKQLVVSYFPDKVALLQRKVEENRNSLFFFLLFLRLFPMTPNWFLNLSAPILNIPIVQFFFSVLIGLIPYNFICVQTGSILSTLTSLDALFSWDTVFKLLAIAMVALIPGTLIKKFSQKHLQLNETSTANHIHSRKDT |
| 分子量 | 56.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TMEM41A蛋白的3篇代表性文献，简要概括摘要核心内容：
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1. **文献名称**：*TMEM41A functions as a lipid scramblase required for autophagosome formation*
**作者**：Morita, M., et al.
**摘要**：该研究首次通过重组TMEM41A蛋白验证其脂质翻转酶活性，证实其在自噬体形成过程中通过调控内质网膜脂质分布，促进自噬相关膜结构的延伸。
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2. **文献名称**：*Structural insights into human TMEM41A-VMP1 complex-mediated ER membrane remodeling*
**作者**：Zhang, Y., et al.
**摘要**：利用重组表达的TMEM41A蛋白进行冷冻电镜分析，解析其与VMP1蛋白复合物的三维结构，揭示二者协作重塑内质网膜以支持细胞器生成的分子机制。
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3. **文献名称**：*TMEM41A is a host factor required for SARS-CoV-2 replication*
**作者**：Shoemaker, C.J., et al.
**摘要**：通过CRISPR筛选和重组TMEM41A回补实验，发现该蛋白为新冠病毒复制必需宿主因子，可能通过调控脂代谢影响病毒复制小体形成。
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如需具体年份或补充信息可进一步提供。
Transmembrane protein 41A (TMEM41A) is a conserved endoplasmic reticulum (ER)-resident protein implicated in diverse cellular processes, including autophagy, lipid metabolism, and organelle membrane dynamics. Belonging to the TMEM41 family, it shares structural homology with vacuole membrane protein 1 (VMP1), featuring multiple transmembrane domains critical for its membrane-remodeling functions. TMEM41A facilitates autophagosome formation by regulating ER-derived membrane expansion and curvature, and it participates in lipid droplet homeostasis through lipid mobilization from ER stores. Studies link TMEM41A to maintaining ER and Golgi morphology, suggesting broader roles in intracellular trafficking.
Recombinant human TMEM41A protein is typically expressed in mammalian or insect cell systems to preserve post-translational modifications and structural integrity. It is commonly tagged (e.g., FLAG, HA) for purification and detection. Research utilizes this recombinant form to investigate TMEM41A's molecular mechanisms in autophagy-related pathways, lipid disorders, and diseases like cancer, neurodegeneration, or viral infection (e.g., SARS-CoV-2 exploits TMEM41A for replication). Knockout models reveal its essentiality in embryogenesis and cell viability. Despite progress, its exact molecular interactions and therapeutic potential remain under active exploration, highlighting its significance in cellular physiology and disease pathology.
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