| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TMEM38B |
| Uniprot No | Q9NVV0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-291 aa |
| 活性数据 | MDSPWDELALAFSRTSMFPFFDIAHYLVSVMAVKRQPGAAALAWKNPISSWFTAMLHCFGGGILSCLLLAEPPLKFLANHTNILLASSIWYITFFCPHDLVSQGYSYLPVQLLASGMKEVTRTWKIVGGVTHANSYYKNGWIVMIAIGWARGAGGTIITNFERLVKGDWKPEGDEWLKMSYPAKVTLLGSVIFTFQHTQHLAISKHNLMFLYTIFIVATKITMMTTQTSTMTFAPFEDTLSWMLFGWQQPFSSCEKKSEAKSPSNGVGSLASKPVDVASDNVKKKHTKKNE |
| 分子量 | 58.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TMEM38B蛋白的参考文献示例(**注：文献为假设性示例，实际引用需核实真实来源**)：
1. **文献名称**：*Structural and Functional Analysis of Recombinant Human TMEM38B as a TRIC-B Channel*
**作者**：Zhao et al. (2017)
**摘要**：通过冷冻电镜解析重组人TMEM38B蛋白的三维结构，揭示其作为三聚体阳离子通道(TRIC-B)的分子机制，阐明其在调控内质网钙释放中的关键作用。
2. **文献名称**：*TMEM38B Mutations Disrupt Calcium Homeostasis in Osteogenesis Imperfecta*
**作者**：Yazawa et al. (2016)
**摘要**：发现TMEM38B基因突变导致隐性遗传性成骨不全症，通过体外实验证明重组TMEM38B蛋白缺失会损害成骨细胞钙信号传导，降低骨基质矿化能力。
3. **文献名称**：*Recombinant TMEM38B Expression Rescues Cellular Calcium Defects in Knockout Models*
**作者**：Smith et al. (2022)
**摘要**：成功在哺乳动物细胞中表达功能性重组TMEM38B蛋白，并在基因敲除模型中验证其恢复钙稳态的能力，为基因治疗提供潜在策略。
4. **文献名称**：*TRIC-B Channels Modulate ER Calcium Release and Muscle Differentiation*
**作者**：Volodarsky & Marini (2020)
**摘要**：研究重组TMEM38B蛋白在细胞分化中的作用，证实其通过调节内质网-线粒体钙信号通路影响肌细胞分化进程。
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**提示**：以上为示例文献，实际研究请参考权威数据库(如PubMed)的收录论文。真实文献可能包括：
- *Shaheen et al. (2012)* 在*PLOS Genetics*报道TMEM38B突变与成骨不全症的关联。
- *Yamazaki et al. (2009)* 在*J Biol Chem*分析TRIC-B单通道特性。
Transmembrane protein 38B (TMEM38B) is a conserved multi-spanning membrane protein encoded by the TMEM38B gene, located on human chromosome 9q31.2. It belongs to the TRIC (TRImolecular Complex) family, functioning as a monovalent cation channel implicated in intracellular calcium homeostasis. TMEM38B forms heteromeric complexes with TRIC-A (TMEM38A) primarily in the endoplasmic/sarcoplasmic reticulum (ER/SR), where it facilitates counterion movement during Ca²⁺ release from intracellular stores. This process is critical for stabilizing membrane potential and ensuring efficient Ca²⁺ signaling in excitable cells, particularly in muscles and neurons.
Recombinant human TMEM38B protein is typically expressed in heterologous systems (e.g., HEK293 or insect cells) for structural and functional studies. Its 295-amino-acid sequence features three predicted transmembrane domains, with both N- and C-termini oriented toward the cytoplasm. Research highlights its role in bone formation, as TMEM38B mutations are linked to autosomal recessive osteogenesis imperfecta type XIV, characterized by collagen defects and skeletal fragility. Knockout models demonstrate abnormal ER Ca²⁺ handling, impaired osteoblast differentiation, and muscle weakness. Recent studies also suggest involvement in cardiac and renal functions through Ca²⁺-dependent pathways. TMEM38B’s recombinant forms enable investigations into TRIC channel mechanics, calcium-associated diseases, and potential therapeutic targets.
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