| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TMEM24 |
| Uniprot No | Q80X80 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-706 aa |
| 活性数据 | MDPGWGQRDVGWAALLILFAASLLTVFAWLLQYARGLWLARARGDRGPGPALAGEPAGSLRELGVWRSLLRLRATRAGAAEEPGVRGLLASLFAFKSFRENWQRAWVRALNEQACRNGSSIQIAFEEVPQLPPRASISHVTCVDQSEHTMVLRCQLSAEEVRFPVSVTQQSPAAVSMETYHVTLTLPPTQLEVNLEEIPGEGLLISWAFTDRPDLSLTVLPKLQARERGEEQVELSTIEELIKDAIVSTQPAMMVNLRACSAPGGLVPSEKPPMMPQAQPAIPRPNRLFLRQLRASHLGNELEGTEELCCVAELDNPMQQKWTKPARAGSEVEWTEDLALDLGPQSRELTLKVLRSSSCGDTELLGQATLPVGSPSRPLSRRQLCPLTPGPGKALGPAATMAVELHYEEGSPRNLGTPTSSTPRPSITPTKKIELDRTIMPDGTIVTTVTTVQSRPRIDGKLDSPSRSPSKVEVTEKTTTVLSESSGPSNTSHSSSRDSHLSNGLDPVAETAIRQLTEPSGRVAKKTPTKRSTLIISGVSKVPIAQDELALSLGYAASLEASVQDDAGTSGGPSSPPSDPPAMSPGPLDALSSPTSVQEADETTRSDISERPSVDDIESETGSTGALETRSLKDHKVSFLRSGTKLIFRRRPRQKEAGLSQSHDDLSNATATPSVRKKAGSFSRRLIKRFSFKSKPKANGNPSPQL |
| 分子量 | 102.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **文献名称**: "TMEM24 facilitates membrane trafficking by coupling synthesis of phosphatidylinositol 4.5-bisphosphate to ER–plasma membrane contact sites"
**作者**: Kim YJ, Guzman-Hernandez ML, Balla T
**摘要**: 该研究揭示了TMEM24在内质网与质膜接触部位的作用，通过调节磷脂酰肌醇4.5-二磷酸(PIP2)的合成，参与细胞内的钙信号传导和膜运输过程，维持细胞动态平衡。
2. **文献名称**: "TMEM24 is a regulator of glucose-stimulated insulin secretion and β-cell endoplasmic reticulum Ca²⁺ homeostasis"
**作者**: Zhang Y, Yu J, Chen Y, et al.
**摘要**: 研究发现TMEM24通过调控β细胞内质网钙稳态，影响葡萄糖刺激下的胰岛素分泌，提示其可能在糖尿病病理生理中发挥关键作用。
3. **文献名称**: "Structural basis for the role of TMEM24 in coordinating lipid transport at ER-mitochondria contact sites"
**作者**: Liu X, Li D, Prinz WA
**摘要**: 利用冷冻电镜技术解析TMEM24的结构，揭示其作为脂质转运蛋白的功能机制，通过介导线粒体与内质网接触，参与脂质代谢与细胞能量平衡调控。
4. **文献名称**: "TMEM24 modulates autophagy via PI3P regulation and is linked to neurodegenerative disorders"
**作者**: Sharma M, Subramanian N, Tang D
**摘要**: 研究表明TMEM24通过调控磷脂酰肌醇3-磷酸(PI3P)水平影响自噬过程，其功能异常与阿尔茨海默病等神经退行性疾病相关，为治疗提供了潜在靶点。
以上文献涵盖了TMEM24在膜运输、代谢调节、结构机制及疾病关联中的多维度研究。
Transmembrane protein 24 (TMEM24) is a eukaryotic protein primarily localized to the endoplasmic reticulum (ER) and plasma membrane contact sites. Identified for its role in lipid homeostasis, it contains an N-terminal SMP (synaptotagmin-like mitochondrial-lipid-binding) domain, a central intrinsically disordered region, and a C-terminal transmembrane anchor. TMEM24 facilitates non-vesicular lipid transport between the ER and plasma membrane, particularly phosphoinositides, which are critical for cellular signaling and membrane dynamics.
Research highlights its calcium-regulated function: TMEM24 dissociates from the ER under low cytoplasmic Ca²⁺ levels to tether ER-plasma membrane junctions, enabling lipid exchange during signaling events. This process supports sustained phosphatidylinositol 4-phosphate (PI4P) synthesis, crucial for insulin secretion in pancreatic β-cells and synaptic transmission in neurons. Dysregulation of TMEM24 has been implicated in metabolic and neurological disorders, including diabetes and bipolar disorder, due to its impact on lipid-dependent signaling pathways.
Recombinant human TMEM24 protein is widely used to study lipid transfer mechanisms, membrane contact site biology, and calcium signaling crosstalk. Its structural versatility and role in synaptamide biosynthesis further underscore its therapeutic potential. Current studies employ CRISPR, fluorescence imaging, and lipidomic approaches to explore TMEM24’s physiological relevance and disease connections. (Word count: 200)
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