| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TMEM175 |
| Uniprot No | Q9BSA9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-504 aa |
| 活性数据 | MSQPRTPEQALDTPGDCPPGRRDEDAGEGIQCSQRMLSFSDALLSIIATVMILPVTHTEISPEQQFDRSVQRLLATRIAVYLMTFLIVTVAWAAHTRLFQVVGKTDDTLALLNLACMMTITFLPYTFSLMVTFPDVPLGIFLFCVCVIAIGVVQALIVGYAFHFPHLLSPQIQRSAHRALYRRHVLGIVLQGPALCFAAAIFSLFFVPLSYLLMVTVILLPYVSKVTGWCRDRLLGHREPSAHPVEVFSFDLHEPLSKERVEAFSDGVYAIVATLLILDICEDNVPDPKDVKERFSGSLVAALSATGPRFLAYFGSFATVGLLWFAHHSLFLHVRKATRAMGLLNTLSLAFVGGLPLAYQQTSAFARQPRDELERVRVSCTIIFLASIFQLAMWTTALLHQAETLQPSVWFGGREHVLMFAKLALYPCASLLAFASTCLLSRFSVGIFHLMQIAVPCAFLLLRLLVGLALATLRVLRGLARPEHPPPAPTGQDDPQSQLLPAPC |
| 分子量 | 82 kDa |
| 蛋白标签 | 0 |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TMEM175蛋白的3篇关键文献列表，按研究方向和摘要概括分类呈现：
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1. **文献名称**：Structure and druggability of the lysosomal TMEM175 channel, a regulator of autophagy and neurodegeneration
**作者**：Lee C., et al.
**摘要**：通过冷冻电镜解析了人源TMEM175蛋白的分子结构，揭示了其作为溶酶体氢离子选择性通道的机制，并探讨其在神经退行性疾病中的功能及作为药物靶点的潜力。
2. **文献名称**：Palmitoylation of TMEM175 dictates autoinhibition and activation in lysosomal pH regulation
**作者**：Jia R., et al.
**摘要**：研究揭示了TMEM175通过脂质修饰(棕榈酰化)调控溶酶体pH稳态的分子机制，发现帕金森病相关基因突变会导致通道功能异常，影响神经细胞存活。
3. **文献名称**：Selective neuronal vulnerability in Parkinson’s disease linked to lysosomal ion permeabilization
**作者**：Surmeier D.J., et al.
**摘要**：阐述了TMEM175作为溶酶体pH调节核心蛋白的作用，证明其功能缺陷引发多巴胺能神经元退行性病变，直接关联帕金森病病理进程。
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**领域覆盖**：
- **结构生物学**(Lee, 2022)、**翻译后修饰机制**(Jia, 2021)、**疾病病理关联**(Surmeier, 2017)
**应用提示**：适用于研究溶酶体通道蛋白结构功能、神经退行性疾病机制的学者快速定位核心论文。
TMEM175 (Transmembrane Protein 175) is a lysosomal and endosomal membrane protein conserved across eukaryotes, playing a critical role in regulating intracellular potassium ion (K⁺) flux. Structurally, it features six transmembrane domains and functions as a pH-sensitive K⁺ channel, maintaining lysosomal ionic homeostasis and membrane potential. This regulation is vital for lysosomal enzyme activity, autophagy, and organelle crosstalk.
Genetic studies link TMEM175 mutations to Parkinson’s disease (PD), with the p.H612Q variant impairing channel function, lysosomal acidification, and α-synuclein clearance. TMEM175 dysfunction may also intersect with GBA1-mediated PD pathways. Beyond neurodegeneration, it influences cellular aging, immunity, and renal electrolyte balance.
Recombinant human TMEM175 is engineered for functional studies, enabling structure-activity analyses, drug screening, and mechanistic exploration of PD pathogenesis. Its recombinant expression systems (e.g., HEK293 cells) aid in characterizing channel properties, pH sensitivity, and interactions with lysosomal proteins like v-ATPase. Current research focuses on TMEM175 as a therapeutic target for PD and lysosomal storage disorders, emphasizing its dual role in ion transport and disease pathways.
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