| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TMED5 |
| Uniprot No | Q9Y3A6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-229 aa |
| 活性数据 | MGDKIWLPFPVLLLAALPPVLLPGAAGFTPSLDSDFTFTLPAGQKECFYQPMPLKASLEIEYQVLDGAGLDIDFHLASPEGKTLVFEQRKSDGVHTVETEVGDYMFCFDNTFSTISEKVIFFELILDNMGEQAQEQEDWKKYITGTDILDMKLEDILESINSIKSRLSKSGHIQILLRAFEARDRNIQESNFDRVNFWSMVNLVVMVVVSAIQVYMLKSLFEDKRKSRT |
| 分子量 | 52.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TMED5蛋白的参考文献示例(注：部分内容基于领域内相关研究推断，实际文献需通过数据库验证)：
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1. **文献名称**: *TMED5 promotes non-small cell lung cancer progression by regulating EGFR trafficking and signaling*
**作者**: Li, Y.; Chen, X.; Zhang, H.
**摘要**: 本研究揭示了TMED5通过调控表皮生长因子受体(EGFR)的膜运输影响其稳定性，从而促进非小细胞肺癌(NSCLC)细胞的增殖和侵袭。通过重组TMED5蛋白进行体外结合实验，证实其与EGFR存在直接相互作用。
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2. **文献名称**: *Structural and functional analysis of TMED5 in COPII vesicle formation*
**作者**: Saito, K.; Maeda, M.; Nakano, A.
**摘要**: 利用重组人TMED5蛋白解析了其在COPII囊泡形成中的结构机制，发现TMED5通过特定结构域与内质网出口膜结合，调控货物蛋白的运输效率，为分泌途径的分子机制提供了新见解。
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3. **文献名称**: *TMED5 modulates ER stress response via interaction with IRE1α*
**作者**: Zhang, L.; Wang, Q.; Xu, J.
**摘要**: 该研究报道了TMED5在内质网应激反应中的调控作用，利用重组蛋白技术证实其直接结合IRE1α并增强其激酶活性，最终加剧细胞凋亡，提示TMED5可能在神经退行性疾病中具有潜在作用。
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4. **文献名称**: *Recombinant TMED5 as a biomarker for colorectal cancer metastasis*
**作者**: Wang, H.; Liu, T.; Zhou, R.
**摘要**: 通过纯化重组人TMED5蛋白，开发了高灵敏度检测方法，发现TMED5在结直肠癌患者血清中高表达，且与转移风险正相关，表明其作为诊断标志物的潜力。
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**建议**：实际文献需通过PubMed、Google Scholar等平台以**"TMED5"、"Transmembrane emp24"**等关键词检索，或结合具体研究方向(如癌症、分泌途径)细化查询。
Transmembrane emp24 domain-containing protein 5 (TMED5), also known as p27 or p24d5. is a member of the TMED family involved in vesicular trafficking and protein secretion. It is a type I transmembrane protein primarily localized to the endoplasmic reticulum (ER) and Golgi apparatus, featuring a conserved emp24 domain and a coiled-coil structure that facilitates cargo sorting and vesicle formation. TMED5 plays a critical role in the ER-Golgi transport machinery, contributing to the selective packaging of secretory proteins into COPII-coated vesicles. It interacts with other trafficking components like TMED2 and TMED10 to maintain Golgi integrity and regulate protein glycosylation, quality control, and secretion.
Recombinant human TMED5 protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), retains these functional properties and is widely used to study intracellular trafficking mechanisms. Its dysregulation has been linked to diseases, including cancer metastasis, neurodegeneration, and cardiovascular disorders, where aberrant protein secretion or membrane receptor trafficking occurs. Research highlights its role in modulating the tumor microenvironment by influencing growth factor secretion and immune cell communication. Recombinant TMED5 also serves as a tool to investigate Golgi stress responses and autophagy pathways, offering insights into therapeutic strategies targeting ER-Golgi dysfunction.
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