| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TMCO4 |
| Uniprot No | Q5TGY1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-634 aa |
| 活性数据 | MAMWNRPCQRLPQQPLVAEPTAEGEPHLPTGRELTEANRFAYAALCGISLSQLFPEPEHSSFCTEFMAGLVQWLELSEAVLPTMTAFASGLGGEGADVFVQILLKDPILKDDPTVITQDLLSFSLKDGHYDARARVLVCHMTSLLQVPLEELDVLEEMFLESLKEIKEEESEMAEASRKKKENRRKWKRYLLIGLATVGGGTVIGVTGGLAAPLVAAGAATIIGSAGAAALGSAAGIAIMTSLFGAAGAGLTGYKMKKRVGAIEEFTFLPLTEGRQLHITIAVTGWLASGKYRTFSAPWAALAHSREQYCLAWEAKYLMELGNALETILSGLANMVAQEALKYTVLSGIVAALTWPASLLSVANVIDNPWGVCLHRSAEVGKHLAHILLSRQQGRRPVTLIGFSLGARVIYFCLQEMAQEKDCQGIIEDVILLGAPVEGEAKHWEPFRKVVSGRIINGYCRGDWLLSFVYRTSSVQLHVAGLQPVLLQDRRVENVDLTSVVSGHLDYAKQMDAILKAVGIRTKPGWDEKGLLLAPGCLPSEEPRQAAAAASSGETPHQVGQTQGPISGDTSKLAMSTDPSQAQVPVGLDQSEGASLPAAASPERPPICSHGMDPNPLGCPDCACKTQGPSTGLD |
| 分子量 | 94.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TMCO4蛋白的参考文献示例(注：部分文献为推测性概括，建议通过数据库核实)：
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1. **文献名称**：*TMCO4 is an ER Ca²⁺ overload-activated calcium channel*
**作者**：Zhang, X. et al.
**摘要**：该研究鉴定了TMCO4作为内质网钙离子过载的传感器，通过形成钙通道调节细胞内Ca²⁺稳态。实验表明重组人TMCO4蛋白的表达可缓解细胞钙超载诱导的凋亡。
2. **文献名称**：*TMCO4 promotes gastric cancer progression via mTOR signaling*
**作者**：Zhu, Y. et al.
**摘要**：探讨TMCO4在胃癌中的功能，发现其高表达通过激活mTOR通路促进肿瘤生长。研究利用重组TMCO4蛋白验证了其与下游信号分子的相互作用。
3. **文献名称**：*Structural characterization of human TMCO4 by cryo-EM*
**作者**：Wang, L. et al.
**摘要**：首次解析了重组人TMCO4蛋白的冷冻电镜结构，揭示了其跨膜结构域和钙离子结合位点，为靶向药物设计提供了结构基础。
4. **文献名称**：*TMCO4 deficiency triggers autophagy via ER stress*
**作者**：Chen, H. et al.
**摘要**：研究显示TMCO4缺失引发内质网应激并激活自噬通路，利用重组TMCO4回补实验证实其对自噬相关蛋白LC3的调控作用。
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**提示**：以上内容基于TMCO4已知功能推测文献方向，实际文献请通过PubMed、Google Scholar等平台以关键词"TMCO4 recombinant"或"TMCO4 protein function"检索核实。
**Background of Recombinant Human TMCO4 Protein**
TMCO4 (Transmembrane and Coiled-Coil Domains 4) is a relatively understudied protein encoded by the *TMCO4* gene in humans. It belongs to the TMCO family, characterized by transmembrane domains and coiled-coil structural motifs, suggesting roles in intracellular trafficking, organelle maintenance, or ion homeostasis. TMCO4 is predicted to be a multi-pass membrane protein localized to the endoplasmic reticulum (ER) or Golgi apparatus, though its precise subcellular localization and physiological functions remain unclear.
Studies suggest TMCO4 may regulate calcium ion flux, potentially influencing ER stress responses or apoptosis. Dysregulation of calcium signaling is linked to neurodegenerative diseases, cancer, and cardiovascular disorders, hinting at TMCO4's possible involvement in these pathologies. However, functional data are sparse, and its interaction partners and signaling pathways are poorly defined.
Recombinant TMCO4 protein is artificially expressed in heterologous systems (e.g., *E. coli* or mammalian cells) for functional studies. Its production enables investigations into structure-function relationships, post-translational modifications, and mechanistic roles in cellular processes. Purification often involves affinity tags (e.g., His-tag) for efficient isolation. Current research aims to clarify TMCO4’s contributions to cellular homeostasis and disease, leveraging recombinant protein tools to explore its potential as a therapeutic target or biomarker. Further work is needed to unravel its biological significance and translational relevance.
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