| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TMC7 |
| Uniprot No | Q7Z402 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-723 aa |
| 活性数据 | MSESSGSALQPGRPSRQQAVHPENLSLDSSCFSSPPVNFLQELPSYRSIARRRTTVHSRDKQSGTLLKPTDSYSSQLEDRIAENLSSHSLRNYALNISEKRRLRDIQETQMKYLSEWDQWKRYSSKSWKRFLEKAREMTTHLELWREDIRSIEGKFGTGIQSYFSFLRFLVLLNLVIFLIIFMLVLLPVLLTKYKITNSSFVLIPFKDMDKQCTVYPVSSSGLIYFYSYIIDLLSGTGFLEETSLFYGHYTIDEVKFQNFTYDLPLAYLLSTIASLALSLLWIVKRSVEGFKINLIRSEEHFQSYCNKIFAGWDFCITNRSMADLKHSSLRYELRADLEEERMRQKIAERTSEETIRIYSLRLFLNCIVLAVLGACFYAIYVATVFSQEHMKKEIDKMVFGENLFILYLPSIVITLANFITPMIFAKIIRYEDYSPGFEIRLTILRCVFMRLATICVLVFTLGSKITSCDDDTCDLCGYNQKLYPCWETQVGQEMYKLMIFDFIIILAVTLFVDFPRKLLVTYCSSCKLIQCWGQQEFAIPDNVLGIVYGQTICWIGAFFSPLLPAIATLKFIIIFYVKEWSLLYTCRPSPRPFRASNSNFFFLLVLLIGLCLAIIPLTISISRIPSSKACGPFTNFNTTWEVIPKTVSTFPSSLQSFIHGVTSEAFAVPFFMIICLIMFYFIALAGAHKRVVIQLREQLSLESRDKCYLIQKLTEAQRDMRN |
| 分子量 | 110 kDa |
| 蛋白标签 | 0 |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于重组人跨膜通道样蛋白7(TMC7)的研究目前较为有限，以下为模拟的示例参考文献格式(可能不对应实际发表的文献，建议通过专业数据库进一步核实)：
---
1. **文献名称**：*Functional Characterization of Recombinant Human TMC7 as a Calcium-Permeable Ion Channel*
**作者**：Li X et al.
**摘要**：本研究首次成功在大肠杆菌系统中表达并纯化了重组人TMC7蛋白，功能实验表明其在HEK293细胞膜上形成钙离子通道，可能参与细胞内钙信号调节。
2. **文献名称**：*TMC7 Regulates Lysosomal pH via Proton Transport in Human Cells*
**作者**：Zhang Y et al.
**摘要**：通过重组TMC7蛋白的体外重建实验，发现其在溶酶体膜中发挥质子转运作用，影响溶酶体酸性环境维持，为相关代谢疾病提供机制线索。
3. **文献名称**：*Structural Insights into TMC7 by Cryo-EM: Implications for Channel Gating*
**作者**：Wang H et al.
**摘要**：利用冷冻电镜解析了重组人TMC7蛋白的高分辨率结构，揭示了其独特的跨膜螺旋构象及潜在的离子选择性过滤机制。
---
**建议**：
若需真实文献，请核查以下资源：
- **PubMed**(https://pubmed.ncbi.nlm.nih.gov)
- **UniProt**(搜索TMC7以获取功能注释：https://www.uniprot.org)
- **Google Scholar**(关键词："recombinant human TMC7" / "TMC7 ion channel")
- 确认蛋白名称拼写准确性(TMC7或可能类似蛋白如TMC6、TMC8已被研究)。
**Background of Recombinant Human TMC7 Protein**
Transmembrane Channel-like 7 (TMC7) is a poorly characterized member of the TMC protein family, which shares structural homology with ion channels or transporters. Predicted to contain six transmembrane domains, TMC7 is postulated to play roles in cellular ion homeostasis or membrane-associated processes, though its precise biological functions remain unclear. Unlike its well-studied relatives (e.g., TMC1/TMC2 in auditory transduction), TMC7 has limited research, with initial studies linking it to spermiogenesis and testis-specific expression, suggesting potential roles in male fertility.
Recombinant human TMC7 protein is synthesized using expression systems like *E. coli* or mammalian cells to enable functional and structural studies. Its recombinant form facilitates investigations into substrate interactions, enzymatic properties, or ion channel activity. Notably, TMC7’s genetic locus overlaps with disease-associated regions, including cancer and skin disorders, hinting at broader physiological implications. Current research focuses on resolving its molecular mechanisms, binding partners, and relevance to human pathologies. Despite its enigmatic nature, TMC7 represents a compelling target for elucidating novel pathways in cellular regulation and disease. Further studies are required to validate its hypothesized functions and therapeutic potential.
×
