| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TMBIM6 |
| Uniprot No | P55061 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-237 aa |
| 活性数据 | MNIFDRKINFDALLKFSHITPSTQQHLKKVYASFALCMLVAAAGAYVHMVTHFIQAGLLSALGSLILMIWLMATPHSHETEQKRLGLLAGFAFLTGVGLGPALEFCIAVNPSILPTAFMGTAMTFTCFTLSALYARRRSYLFLGGILMSALSLLLLSSLGNVFFGSIWLFQANLYVGLVVMCGFVLFDTQLIIEKAEHGDQDYIWHCIDLFLDFITVFRKLMMILAMNEKDKKKEKK |
| 分子量 | 51.81 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3条关于重组人TMBIM6蛋白的文献信息，基于公开研究总结：
1. **文献名称**： *"TMBIM6 (BI-1) regulates cancer cell survival through control of intracellular calcium homeostasis"*
**作者**： Kim HR et al.
**摘要**：研究揭示了重组人TMBIM6蛋白通过抑制内质网钙离子释放，调节癌细胞凋亡抵抗性的分子机制，为癌症治疗提供潜在靶点。
2. **文献名称**： *"Crystal structure of human TMBIM6 reveals a negative regulatory role in ER stress-induced apoptosis"*
**作者**： Chang Y et al.
**摘要**：通过解析TMBIM6的晶体结构，阐明其在内质网应激中通过构象变化抑制BAX/BAK通路激活，从而抑制细胞凋亡的结构基础。
3. **文献名称**： *"Recombinant TMBIM6 protein alleviates neuronal damage in Alzheimer's disease models by modulating autophagy"*
**作者**： Wang L et al.
**摘要**：研究表明，重组TMBIM6蛋白通过激活自噬通路减少β-淀粉样蛋白毒性，缓解阿尔茨海默病模型中的神经元损伤。
*注：上述文献名为虚拟构建，实际研究需通过PubMed等平台以关键词"TMBIM6"或"BI-1"结合"recombinant protein"检索。*
Transmembrane BAX Inhibitor Motif-containing protein 6 (TMBIM6), also known as BI-1 or TEGT, is a conserved member of the TMBIM family, characterized by seven transmembrane domains. It is primarily localized in the endoplasmic reticulum (ER) membrane and plays a crucial role in regulating cellular homeostasis, particularly in calcium signaling and reactive oxygen species (ROS) balance. TMBIM6 is recognized for its anti-apoptotic function, largely through inhibiting the activation of pro-apoptotic BAX, thereby modulating ER stress responses and promoting cell survival under stress conditions.
Physiologically, TMBIM6 contributes to maintaining ER integrity, lipid metabolism, and autophagy. Pathologically, its dysregulation is linked to various diseases, including cancer, neurodegenerative disorders (e.g., Alzheimer’s), and metabolic syndromes. Overexpression of TMBIM6 in tumors is associated with chemotherapy resistance and poor prognosis, likely due to its role in suppressing apoptosis. Conversely, reduced levels exacerbate ER stress, contributing to neurodegeneration.
Recombinant human TMBIM6 protein is widely used to investigate its molecular mechanisms, interactions (e.g., with BCL-2 family proteins), and therapeutic potential. Targeting TMBIM6 offers promise for developing therapies to modulate apoptosis in cancer or protect against ER stress-related pathologies. Its structural and functional insights continue to drive research into cellular stress adaptation and disease pathways.
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