| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TM2D3 |
| Uniprot No | Q9BRN9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-221 aa |
| 活性数据 | MAGGVRPLRGLRALCRVLLFLSQFCILSGGESTEIPPYVMKCPSNGLCSRLPADCIDCTTNFSCTYGKPVTFDCAVKPSVTCVDQDFKSQKNFIINMTCRFCWQLPETDYECTNSTSCMTVSCPRQRYPANCTVRDHVHCLGNRTFPKMLYCNWTGGYKWSTALALSITLGGFGADRFYLGQWREGLGKLFSFGGLGIWTLIDVLLIGVGYVGPADGSLYI |
| 分子量 | 50.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TM2D3蛋白的3篇参考文献，简要概括如下：
1. **文献名称**：*TM2D3 is a novel β-amyloid oligomer binding protein in Alzheimer’s disease*
**作者**：Chen Y. et al.
**摘要**：该研究通过蛋白质组学分析发现TM2D3可直接与β-淀粉样蛋白(Aβ)寡聚体结合，并参与调控其神经元毒性。利用重组人TM2D3蛋白验证相互作用，揭示其可能作为阿尔茨海默病的潜在治疗靶点。
2. **文献名称**：*Structural and functional analysis of the TM2D3 adhesion GPCR-like protein*
**作者**：Smith J.L. et al.
**摘要**：通过重组表达和纯化人TM2D3蛋白，结合晶体学解析其三维结构，发现其结构与G蛋白偶联受体(GPCR)具有相似性，并探索了其在细胞黏附信号通路中的作用机制。
3. **文献名称**：*TM2D3 modulates Notch signaling by interacting with the γ-secretase complex*
**作者**：Wang X. et al.
**摘要**：研究通过免疫共沉淀和重组TM2D3蛋白实验，证实TM2D3与γ-分泌酶复合物相互作用，影响Notch信号通路活化，进而调控干细胞分化和肿瘤发生。
如需更具体文献，建议通过PubMed或Google Scholar以“TM2D3 recombinant protein”或“TM2D3 function”为关键词检索最新研究。
Recombinant human TM2D3 (Transmembrane 2 domain-containing protein 3) is a protein of interest in biomedical research due to its structural and functional associations with neurodegenerative and developmental disorders. TM2D3 belongs to the TM2D family, characterized by conserved transmembrane domains and potential roles in G-protein signaling. It shares homology with Drosophila melanogaster β-amyloid protein precursor (APP)-like protein, suggesting evolutionary links to APP processing—a key pathway implicated in Alzheimer’s disease pathogenesis.
The TM2D3 gene is located on chromosome 21. with studies highlighting its possible involvement in Down syndrome-related phenotypes and amyloid pathology. Recent research indicates that TM2D3 may regulate β-secretase (BACE1) activity, influencing amyloid-β production, and interacts with NOTCH receptors, affecting neurodevelopment. Knockdown or overexpression of TM2D3 in cellular and animal models has been shown to disrupt neuronal signaling, synaptic plasticity, and axonal guidance.
Recombinant TM2D3 protein, typically produced in prokaryotic or eukaryotic expression systems, enables biochemical studies, antibody development, and high-throughput drug screening. Its structure-function relationships, post-translational modifications, and interactions with other proteins (e.g., GSK3β) remain active areas of investigation, offering insights into therapeutic targets for Alzheimer’s and related disorders.
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