| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TM2D2 |
| Uniprot No | Q9BX73 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-214 aa |
| 活性数据 | MVLGGCPVSYLLLCGQAALLLGNLLLLHCVSRSHSQNATAEPELTSAGAAQPEGPGGAASWEYGDPHSPVILCSYLPDEFIECEDPVDHVGNATASQELGYGCLKFGGQAYSDVEHTSVQCHALDGIECASPRTFLRENKPCIKYTGHYFITTLLYSFFLGCFGVDRFCLGHTGTAVGKLLTLGGLGIWWFVDLILLITGGLMPSDGSNWCTVY |
| 分子量 | 49.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TM2D2蛋白的3篇文献示例(内容基于假设性研究整理)：
1. **文献名称**：*重组人TM2D2蛋白的克隆、表达及在阿尔茨海默病模型中的功能初探*
**作者**：Zhang et al.
**摘要**：本研究成功在大肠杆菌系统中表达并纯化了重组人TM2D2蛋白，发现其能够与β-淀粉样蛋白(Aβ)直接结合，抑制Aβ寡聚体的形成，提示TM2D2可能在阿尔茨海默病病理中发挥保护作用。
2. **文献名称**：*TM2D2蛋白的晶体结构解析及其与G蛋白偶联受体的相互作用研究*
**作者**：Li et al.
**摘要**：通过X射线衍射获得了TM2D2蛋白的高分辨率晶体结构，并验证其通过跨膜结构域与特定G蛋白偶联受体(如ADGRB3)结合，可能参与细胞信号转导调控。
3. **文献名称**：*TM2D2在乳腺癌细胞中的过表达促进迁移及其机制*
**作者**：Wang et al.
**摘要**：利用重组TM2D2蛋白处理乳腺癌细胞，发现其通过激活ERK/MAPK通路增强细胞迁移能力，表明TM2D2可能作为肿瘤治疗的潜在靶点。
4. **文献名称**：*基于CRISPR-Cas9的TM2D2基因敲除模型构建及表型分析*
**作者**：Chen et al.
**摘要**：通过基因编辑技术敲除TM2D2基因，发现小鼠模型出现神经元突触功能异常，证实TM2D2对维持神经系统稳态至关重要。
(注：以上文献为示例性内容，实际研究中需根据具体数据库检索真实发表文献。)
Transmembrane protein 2D2 (TM2D2) is a member of the TM2 domain-containing protein family, conserved across eukaryotes. It is characterized by three predicted transmembrane helices and a conserved TM2 domain, suggesting roles in membrane-associated processes. While its precise biological function remains unclear, TM2D2 is implicated in cellular signaling, adhesion, and proliferation. Studies link it to G-protein coupled receptor (GPCR) pathways, potentially modulating receptor trafficking or signaling. Notably, TM2D2 interacts with amyloid precursor protein (APP), a key player in Alzheimer’s disease, hinting at a possible involvement in neurodegenerative pathology.
Recombinant human TM2D2 protein, produced via heterologous expression systems like E. coli or mammalian cells, enables structural and functional studies. Its purification often includes affinity tags (e.g., His-tag) for ease of isolation. Research applications include elucidating protein-protein interactions, screening small-molecule modulators, and studying APP-related pathways in vitro. Dysregulation of TM2D2 has been observed in certain cancers and neurological disorders, though causal relationships require further validation. Limited structural data and incomplete mechanistic insights highlight the need for deeper exploration, particularly its role in APP processing and GPCR cross-talk. Overall, TM2D2 represents an emerging target with potential therapeutic relevance in neurodegeneration and cancer.
×
