| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TLL2 |
| Uniprot No | Q9Y6L7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1015 aa |
| 活性数据 | MPRATALGALVSLLLLLPLPRGAGGLGERPDATADYSELDGEEGTEQQLEHYHDPCKAAVFWGDIALDEDDLKLFHIDKARDWTKQTVGATGHSTGGLEEQASESSPDTTAMDTGTKEAGKDGRENTTLLHSPGTLHAAAKTFSPRVRRATTSRTERIWPGGVIPYVIGGNFTGSQRAIFKQAMRHWEKHTCVTFIERTDEESFIVFSYRTCGCCSYVGRRGGGPQAISIGKNCDKFGIVAHELGHVVGFWHEHTRPDRDQHVTIIRENIQPGQEYNFLKMEAGEVSSLGETYDFDSIMHYARNTFSRGVFLDTILPRQDDNGVRPTIGQRVRLSQGDIAQARKLYKCPACGETLQDTTGNFSAPGFPNGYPSYSHCVWRISVTPGEKIVLNFTSMDLFKSRLCWYDYVEVRDGYWRKAPLLGRFCGDKIPEPLVSTDSRLWVEFRSSSNILGKGFFAAYEATCGGDMNKDAGQIQSPNYPDDYRPSKECVWRITVSEGFHVGLTFQAFEIERHDSCAYDYLEVRDGPTEESALIGHFCGYEKPEDVKSSSNRLWMKFVSDGSINKAGFAANFFKEVDECSWPDHGGCEHRCVNTLGSYKCACDPGYELAADKKMCEVACGGFITKLNGTITSPGWPKEYPTNKNCVWQVVAPAQYRISLQFEVFELEGNDVCKYDFVEVRSGLSPDAKLHGRFCGSETPEVITSQSNNMRVEFKSDNTVSKRGFRAHFFSDKDECAKDNGGCQHECVNTFGSYLCRCRNGYWLHENGHDCKEAGCAHKISSVEGTLASPNWPDKYPSRRECTWNISSTAGHRVKLTFNEFEIEQHQECAYDHLEMYDGPDSLAPILGRFCGSKKPDPTVASGSSMFLRFYSDASVQRKGFQAVHSTECGGRLKAEVQTKELYSHAQFGDNNYPSEARCDWVIVAEDGYGVELTFRTFEVEEEADCGYDYMEAYDGYDSSAPRLGRFCGSGPLEEIYSAGDSLMIRFRTDDTINKKGFHARYTSTKFQDALHMKK |
| 分子量 | 138.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人TLL2(Tolloid-like 2)蛋白的模拟参考文献示例(请注意，这些为虚构内容，实际文献需通过学术数据库检索)：
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1. **文献名称**: *Structural and Functional Characterization of Recombinant Human TLL2 Protease*
**作者**: Smith A., et al.
**摘要**: 本研究报道了重组人TLL2蛋白的体外表达与纯化方法，揭示了其依赖锌离子的蛋白酶活性，并通过晶体结构分析阐明了底物结合域的关键氨基酸残基，为靶向TLL2的抑制剂设计提供依据。
2. **文献名称**: *TLL2 in Embryonic Development: Role in BMP Signaling Regulation*
**作者**: Chen L., et al.
**摘要**: 通过基因敲除小鼠模型，证明TLL2通过切割BMP(骨形态发生蛋白)拮抗剂调控胚胎心脏发育，重组TLL2蛋白的体外实验验证了其对Chordin蛋白的水解活性及下游信号通路的影响。
3. **文献名称**: *Association of TLL2 Genetic Variants with Hypertension and Cardiac Remodeling*
**作者**: Gupta R., et al.
**摘要**: 临床研究结合重组TLL2功能实验，发现TLL2基因多态性与高血压患者心脏纤维化相关，重组蛋白的高表达促进体外心肌细胞胶原沉积，提示其作为治疗靶点的潜力。
4. **文献名称**: *Expression and Purification of Active Recombinant TLL2 in Insect Cells*
**作者**: Wang Y., et al.
**摘要**: 利用杆状病毒-昆虫细胞系统高效表达可溶性重组TLL2蛋白，优化纯化步骤后获得高活性产物，并证明其对基质金属蛋白酶底物的特异性切割能力。
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**备注**：以上内容为示例性质，真实文献需通过PubMed、Web of Science或Google Scholar检索关键词(如“TLL2 protease”、“recombinant TLL2”等)获取。TLL2相关研究可能涉及胚胎发育、组织纤维化或肿瘤微环境调控等领域。
Recombinant human TLL2 (tolloid-like 2) protein is a metalloprotease belonging to the astacin family, closely related to BMP-1 (bone morphogenetic protein 1) and other tolloid-like proteases. TLL2 is encoded by the *TLL2* gene and plays critical roles in extracellular matrix (ECM) remodeling and growth factor activation. Structurally, it contains a protease domain with catalytic zinc-binding sites, complement C1r/C1s-Uegf-BMP1 (CUB) domains, and epidermal growth factor (EGF)-like domains, enabling interactions with substrates and co-factors.
Biologically, TLL2 processes precursors such as procollagen and prolysyl oxidase, contributing to collagen fibril assembly and ECM maturation. It also cleaves inhibitors of BMP/TGF-β signaling pathways (e.g., chordin), modulating embryonic development, tissue homeostasis, and wound healing. Dysregulation of TLL2 has been linked to fibrosis, cardiovascular disorders, and cancer progression due to its influence on cell differentiation and ECM dynamics.
Recombinant TLL2 is produced via heterologous expression systems (e.g., mammalian or insect cells) to ensure proper post-translational modifications. Its purified form is widely used in biochemical assays, structural studies, and drug discovery to explore protease mechanisms or screen inhibitors. Researchers also investigate its therapeutic potential in diseases involving ECM dysfunction. Despite functional overlaps with BMP-1/TLL1. TLL2 exhibits unique substrate preferences and regulatory roles, underscoring its distinct biological significance. Ongoing studies aim to clarify its tissue-specific functions and interactions within proteolytic networks.
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