| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TLE6 |
| Uniprot No | Q9H808 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-572 aa |
| 活性数据 | MTSRDQPRPK GPPKSTSPCP GISNSESSPT LNYQGILNRL KQFPRFSPHF AAELESIYYS LHKIQQDVAE HHKQIGNVLQ IVESCSQLQG FQSEEVSPAE PASPGTPQQV KDKTLQESSF EDIMATRSSD WLRRPLGEDN QPETQLFWDK EPWFWHDTLT EQLWRIFAGV HDEKAKPRDR QQAPGLGQES KAPGSCDPGT DPCPEDASTP RPPEASSSPP EGSQDRNTSW GVVQEPPGRA SRFLQSISWD PEDFEDAWKR PDALPGQSKR LAVPCKLEKM RILAHGELVL ATAISSFTRH VFTCGRRGIK VWSLTGQVAE DRFPESHLPI QTPGAFLRTC LLSSNSRSLL TGGYNLASVS VWDLAAPSLH VKEQLPCAGL NCQALDANLD ANLAFASFTS GVVRIWDLRD QSVVRDLKGY PDGVKSIVVK GYNIWTGGPD ACLRCWDQRT IMKPLEYQFK SQIMSLSHSP QEDWVLLGMA NGQQWLQSTS GSQRHMVGQK DSVILSVKFS PFGQWWASVG MDDFLGVYSM PAGTKVFEVP EMSPVTCCDV SSNNRLVVTG SGEHASVYQI TY |
| 分子量 | 63.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TLE6蛋白的模拟参考文献(内容基于领域知识，具体文献需自行验证)：
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1. **文献名称**：*The role of TLE6 in human oocyte maturation and preimplantation embryonic development*
**作者**：Wang Y, et al.
**摘要**：研究通过重组表达TLE6蛋白，发现其作为母体效应基因的关键作用，调控卵母细胞成熟及胚胎早期分裂，缺失导致胚胎停育。实验验证了TLE6与卵皮质颗粒蛋白的相互作用。
2. **文献名称**：*Structural and functional analysis of the TLE6 N-terminal domain in Wnt signaling regulation*
**作者**：Smith J, et al.
**摘要**：通过重组人TLE6蛋白的晶体结构解析，揭示其N端结构域如何介导与Wnt通路蛋白的结合，抑制β-catenin转录活性，为癌症治疗提供潜在靶点。
3. **文献名称**：*CRISPR-based screening identifies TLE6 as a critical regulator of pluripotency in human embryonic stem cells*
**作者**：Li H, et al.
**摘要**：利用重组TLE6蛋白进行功能回补实验，证明其在维持胚胎干细胞多能性中的作用，并通过表观遗传调控机制影响Oct4/Sox2表达。
4. **文献名称**：*Recombinant TLE6 protein as a biomarker for female infertility: diagnostic potential and mechanistic insights*
**作者**：Chen L, et al.
**摘要**：开发重组TLE6蛋白检测试剂盒，发现TLE6表达异常与女性不明原因不孕相关，机制涉及胚胎着床过程的信号通路失调。
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**说明**：以上为模拟参考文献，实际引用请通过PubMed、Web of Science等数据库检索确认。建议使用关键词“TLE6 recombinant protein”“human TLE6 function”进一步筛选近五年文献。
Transducin-like enhancer of split 6 (TLE6), a member of the Groucho/TLE family of transcriptional corepressors, plays critical roles in embryonic development and cell signaling regulation. It functions as a key component of the subcortical maternal complex (SCMC) in oocytes and preimplantation embryos, contributing to zygotic genome activation, cell polarity establishment, and early embryonic cell division. Structurally, TLE6 contains conserved WD40 repeat domains that mediate protein-protein interactions, particularly with other SCMC members like PADI6 and NLRP5.
As a maternal-effect gene product, TLE6 is predominantly expressed from the maternal genome during oogenesis. Its dysfunction has been linked to human infertility and early embryonic developmental arrest, with mutations associated with recurrent pregnancy loss and embryonic aneuploidy. Recombinant human TLE6 protein, typically produced in bacterial or mammalian expression systems, enables functional studies of its interactions with binding partners and its regulatory mechanisms in developmental pathways like Wnt and Notch signaling.
Research utilizing recombinant TLE6 has advanced our understanding of its role in maintaining genomic stability, regulating epigenetic modifications, and coordinating cell fate decisions. These studies hold potential for developing diagnostic biomarkers for infertility and therapeutic strategies targeting early embryonic developmental disorders.
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