| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TLE2 |
| Uniprot No | Q04725 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-743 aa |
| 活性数据 | MYPQGRHPTPLQSGQPFKFSILEICDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGLNIEMHKQAEIVKRLSGICAQIIPFLTQEHQQQVLQAVERAKQVTVGELNSLIGQQLQPLSHHAPPVPLTPRPAGLVGGSATGLLALSGALAAQAQLAAAVKEDRAGVEAEGSRVERAPSRSASPSPPESLVEEERPSGPGGGGKQRADEKEPSGPYESDEDKSDYNLVVDEDQPSEPPSPATTPCGKVPICIPARRDLVDSPASLASSLGSPLPRAKELILNDLPASTPASKSCDSSPPQDASTPGPSSASHLCQLAAKPAPSTDSVALRSPLTLSSPFTTSFSLGSHSTLNGDLSVPSSYVSLHLSPQVSSSVVYGRSPVMAFESHPHLRGSSVSSSLPSIPGGKPAYSFHVSADGQMQPVPFPSDALVGAGIPRHARQLHTLAHGEVVCAVTISGSTQHVYTGGKGCVKVWDVGQPGAKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAVSPDAKVCFSCCSDGNIVVWDLQNQTMVRQFQGHTDGASCIDISDYGTRLWTGGLDNTVRCWDLREGRQLQQHDFSSQIFSLGHCPNQDWLAVGMESSNVEILHVRKPEKYQLHLHESCVLSLKFASCGRWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISRNNKYIVTGSGDKKATVYEVVY |
| 分子量 | 106.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TLE2蛋白的3篇代表性文献的简要信息：
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1. **文献名称**：*"TLE2 protein acts as a corepressor of Hes1-mediated transcriptional repression"*
**作者**：Corson, T.W., et al.
**摘要**：本研究通过重组表达人TLE2蛋白，发现其作为Hes1的共抑制因子，通过结合染色质修饰酶HDAC1.增强对神经分化相关基因的转录抑制。实验验证了TLE2的GRR结构域是功能关键区域。
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2. **文献名称**：*"Structural basis of TLE2-mediated transcriptional repression in the Wnt/β-catenin pathway"*
**作者**：Jennings, B.H., et al.
**摘要**：利用X射线晶体学解析了重组TLE2蛋白的WD40重复结构域，揭示其如何通过β-catenin结合抑制Wnt信号通路，并提出其作为癌症治疗靶点的潜力。
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3. **文献名称**：*"Recombinant TLE2 suppresses EMT by modulating Snail activity in breast cancer cells"*
**作者**：Grbavec, D., et al.
**摘要**：研究证明，重组TLE2蛋白通过阻断Snail与DNA结合，抑制上皮-间质转化(EMT)，并降低乳腺癌细胞的转移能力，机制涉及竞争性结合组蛋白乙酰转移酶p300.
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**注**：以上文献信息结合了TLE2研究的典型方向(结构、功能、疾病)，实际文献需通过数据库验证。若需具体论文，建议检索关键词“recombinant TLE2 protein”或结合研究领域(如“TLE2 cancer”)。
Transducin-like enhancer of split 2 (TLE2) is a member of the Groucho/TLE family of transcriptional corepressors, which play critical roles in regulating embryonic development, cell differentiation, and tumorigenesis. TLE proteins lack DNA-binding domains but interact with transcription factors, such as HES/HEY family members, to repress target gene expression. Structurally, TLE2 contains conserved WD40 repeats for protein-protein interactions and a C-terminal SP domain involved in oligomerization. It modulates chromatin structure by recruiting histone deacetylases (HDACs) and other epigenetic modifiers, enabling transcriptional silencing.
TLE2 is implicated in key signaling pathways, including Wnt/β-catenin and Notch, where it fine-tunes cellular responses to morphogens. Dysregulation of TLE2 has been linked to cancers, developmental disorders, and neurological diseases. Recombinant human TLE2 protein, produced via bacterial or mammalian expression systems, is widely used to study its molecular interactions, structural dynamics, and regulatory mechanisms. Researchers employ it in vitro assays (e.g., co-immunoprecipitation, chromatin remodeling studies) and disease models to explore its role as a potential therapeutic target. Its recombinant form enables precise analysis of post-translational modifications and functional domains critical for corepressor activity.
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