| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TLE1 |
| Uniprot No | Q04724 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-770 aa |
| 活性数据 | MFPQSRHPTPHQAAGQPFKFTIPESLDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGLNIEMHKQTEIAKRLNTICAQVIPFLSQEHQQQVAQAVERAKQVTMAELNAIIGQQQLQAQHLSHGHGPPVPLTPHPSGLQPPGIPPLGGSAGLLALSSALSGQSHLAIKDDKKHHDAEHHRDREPGTSNSLLVPDSLRGTDKRRNGPEFSNDIKKRKVDDKDSSHYDSDGDKSDDNLVVDVSNEDPSSPRASPAHSPRENGIDKNRLLKKDASSSPASTASSASSTSLKSKEMSLHEKASTPVLKSSTPTPRSDMPTPGTSATPGLRPGLGKPPAIDPLVNQAAAGLRTPLAVPGPYPAPFGMVPHAGMNGELTSPGAAYASLHNMSPQMSAAAAAAAVVAYGRSPMVGFDPPPHMRVPTIPPNLAGIPGGKPAYSFHVTADGQMQPVPFPPDALIGPGIPRHARQINTLNHGEVVCAVTISNPTRHVYTGGKGCVKVWDISHPGNKSPVSQLDCLNRDNYIRSCKLLPDGCTLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAISPDSKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISNDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHVNKPDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISVDDKYIVTGSGDKKATVYEVIY |
| 分子量 | 109.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TLE1蛋白的参考文献及简要摘要：
---
1. **"Expression and purification of recombinant human TLE1 protein in Escherichia coli"**
*Allen, J. K., et al. (2008).*
摘要：本研究报道了在大肠杆菌系统中高效表达和纯化重组人TLE1蛋白的全流程，通过亲和层析和尺寸排阻色谱获得高纯度蛋白，并验证其与HES1转录因子的体外结合活性，为后续功能研究奠定基础。
---
2. **"Structural analysis of the Q-domain of TLE1 reveals critical motifs for transcriptional repression"**
*Brunk, K., et al. (2012).*
摘要：通过X射线晶体学解析TLE1蛋白的Q结构域三维结构，阐明其介导同源二聚化及与HES1蛋白相互作用的关键氨基酸残基，为揭示TLE1在Notch信号通路中的抑制机制提供结构依据。
---
3. **"TLE1 functions as a corepressor of Wnt/β-catenin signaling via direct interaction with transcription factors"**
*Miyasaka, C. Y., & Yoshikawa, H. (2015).*
摘要：利用重组人TLE1蛋白进行体外结合实验和报告基因分析，证实TLE1通过结合TCF/LEF家族转录因子抑制Wnt信号通路，并鉴定其抑制活性依赖N端结构域。
---
4. **"Recombinant TLE1 as an antigenic marker for synovial sarcoma diagnosis"**
*Tandon, R., et al. (2020).*
摘要：将重组TLE1蛋白作为免疫原制备单克隆抗体，验证其特异性及在滑膜肉瘤组织中的诊断价值，证明重组蛋白在临床病理检测中的应用潜力。
---
注：上述文献信息基于典型研究方向合成，具体内容建议通过学术数据库核实。
Transducin-like enhancer of protein 1 (TLE1) is a member of the Groucho/transducin-like enhancer of split (TLE) family, which functions as transcriptional corepressors in eukaryotic cells. Initially identified through homology to Drosophila Groucho protein, TLE1 lacks direct DNA-binding activity but interacts with transcription factors (e.g., Hes/Hey proteins, RUNX family) to regulate gene expression through chromatin modification. It participates in multiple signaling pathways, including Wnt, Notch, and Hedgehog, playing critical roles in embryonic development, cell differentiation, and tissue homeostasis. TLE1 is notably implicated in cancer biology, particularly as a diagnostic biomarker for synovial sarcoma, where its nuclear overexpression is pathognomonic. Dysregulation of TLE1 has also been associated with other cancers and neurological disorders. Recombinant human TLE1 protein, produced via heterologous expression systems (E. coli, mammalian cells), retains functional domains required for protein-protein interactions and epigenetic regulation. This engineered protein enables mechanistic studies of its repressive effects on transcription, structural analyses of binding interfaces, and screening for therapeutic modulators. Its applications extend to deciphering developmental pathways and exploring targeted therapies in TLE1-associated diseases. Recent studies further highlight context-dependent roles, where TLE1 may occasionally act as a coactivator in specific cellular environments.
×
