| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TIMM9 |
| Uniprot No | Q9Y5J7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-89 aa |
| 活性数据 | AAQIPESDQ IKQFKEFLGT YNKLTETCFL DCVKDFTTRE VKPEETTCSE HCLQKYLKMT QRISMRFQEY HIQQNEALAA KAGLLGQPR |
| 分子量 | 10.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TIMM9蛋白的3篇参考文献及简要摘要：
1. **文献名称**: *The role of Tim9 in the mitochondrial protein import motor*
**作者**: Curran SP, et al.
**摘要**: 研究揭示了重组人TIMM9蛋白在线粒体蛋白质导入中的作用，证实其作为TIM(线粒体内膜转位酶)复合体的关键组分，与TIMM10协同介导前体蛋白的跨膜转运。实验通过体外重组表达TIMM9.分析其与底物蛋白的相互作用机制。
2. **文献名称**: *Structural and functional characterization of recombinant human Tim9/Tim10 complex*
**作者**: Webb CT, et al.
**摘要**: 通过体外重组表达并纯化TIMM9与TIMM10蛋白，解析了该复合物的结构特征，证明其通过锌指结构域维持稳定性，并参与特定线粒体膜间隙前体蛋白的识别与折叠。
3. **文献名称**: *Mutations in TIMM9 cause mitochondrial dysfunction and neurological disorders*
**作者**: Roesch K, et al.
**摘要**: 该研究发现TIMM9基因突变导致重组蛋白功能缺陷，破坏线粒体蛋白稳态并引发神经退行性表型。研究利用重组TIMM9蛋白模型验证了突变对其与膜复合物结合能力的影响。
(注：以上文献信息为示例性内容，实际文献需通过数据库查询具体发表信息。)
TIMM9 (Translocase of Inner Mitochondrial Membrane 9) is a crucial component of the mitochondrial protein import machinery in humans. Belonging to the Tim9-Tim10 complex family, it resides in the mitochondrial intermembrane space, facilitating the transfer of hydrophobic precursor proteins from the outer to inner mitochondrial membrane. This conserved chaperone-like protein forms a hexameric complex with TIMM10. stabilizing precursor proteins during their transit to prevent aggregation.
Recombinant human TIMM9 is produced via heterologous expression systems (e.g., E. coli or yeast) for biochemical and functional studies. Its recombinant form enables research into mitochondrial protein import mechanisms, particularly related to metabolic enzymes and membrane carriers. Structural studies using recombinant TIMM9 have revealed its conserved twin CX3C zinc-binding motifs, critical for maintaining structural stability and client protein interactions.
Dysregulation of TIMM9 is linked to mitochondrial disorders, neurodegenerative diseases, and cancer progression, making it a potential therapeutic target. Recombinant versions are essential tools for investigating mitochondrial dysfunction mechanisms and screening drug candidates. Quality assessments typically include mass spectrometry for sequence validation, circular dichroism for secondary structure analysis, and client protein binding assays to confirm functionality. Current research focuses on its role in metabolic homeostasis and disease-specific protein mislocalization.
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