| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TIMM22 |
| Uniprot No | Q9Y584 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-194 aa |
| 活性数据 | MAAAAPNAGGSAPETAGSAEAPLQYSLLLQYLVGDKRQPRLLEPGSLGGIPSPAKSEEQKMIEKAMESCAFKAALACVGGFVLGGAFGVFTAGIDTNVGFDPKDPYRTPTAKEVLKDMGQRGMSYAKNFAIVGAMFSCTECLIESYRGTSDWKNSVISGCITGGAIGFRAGLKAGAIGCGGFAAFSAAIDYYLR |
| 分子量 | 46.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TIMM22蛋白的3篇代表性文献的示例(内容为虚构示例，仅作格式参考)：
1. **文献名称**: *TIMM22 mediates the assembly of the mitochondrial import machinery for inner membrane proteins*
**作者**: Smith A, et al.
**摘要**: 研究揭示了TIMM22蛋白在线粒体内膜蛋白转运复合体中的核心作用，通过重组人TIMM22蛋白体外实验证实其与Tim54/Tim12的相互作用，并解析了其对多跨膜蛋白靶向的分子机制。
2. **文献名称**: *Structural insights into the TIMM22 complex by cryo-EM*
**作者**: Zhang L, et al.
**摘要**: 利用冷冻电镜技术解析了重组人TIMM22复合物的三维结构，发现其形成亲水性通道并通过静电相互作用识别底物蛋白的跨膜结构域。
3. **文献名称**: *Defects in TIMM22 cause mitochondrial dysfunction and neurodegenerative disorders*
**作者**: Wang Y, et al.
**摘要**: 通过基因编辑细胞模型发现，TIMM22蛋白的缺失导致线粒体膜电位异常，并显著增加神经元细胞凋亡，提示其与帕金森病等神经退行性疾病的潜在关联。
**建议**：请通过PubMed或Google Scholar搜索“TIMM22 protein recombinant human”或“TIMM22 function”获取真实文献。实际研究中建议优先选择近5年、高影响因子期刊的论文。
TIMM22 (Translocase of Inner Mitochondrial Membrane 22) is a crucial protein component of the mitochondrial inner membrane translocase complex. It plays a pivotal role in the import and assembly of integral membrane proteins within the mitochondria, particularly those harboring multiple transmembrane domains, such as carriers for metabolites and components of the electron transport chain.
As part of the TIM22 complex, TIMM22 forms a voltage-activated channel that cooperates with other TIM chaperones (e.g., TIMM9. TIMM10) to recognize and guide precursor proteins synthesized in the cytosol. Unlike the TIM23 complex that handles soluble matrix proteins, TIMM22 specializes in membrane protein integration, relying on the membrane potential for substrate recognition and translocation. Structurally, it contains four conserved transmembrane helices and associates with lipid species like cardiolipin to maintain membrane stability.
Mutations or dysfunction in TIMM22 disrupt mitochondrial proteostasis, impair oxidative phosphorylation, and are linked to neurodegenerative disorders (e.g., Parkinson's disease), metabolic syndromes, and mitochondrial encephalopathies. Its dual role in protein sorting and membrane organization underscores its importance in mitochondrial bioenergetics and cellular health. Research continues to explore its regulatory mechanisms and therapeutic potential in mitochondrial-related pathologies.
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