| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TIMM13 |
| Uniprot No | Q9Y5L4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-95 aa |
| 活性数据 | MEGGFGSDFG GSGSGKLDPG LIMEQVKVQI AVANAQELLQ RMTDKCFRKC IGKPGGSLDN SEQKCIAMCM DRYMDAWNTV SRAYNSRLQR ERANM |
| 分子量 | 10.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TIMM13蛋白的3篇参考文献的简要信息：
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1. **文献名称**: "The Tim8-Tim13 complex mediates mitochondrial protein import"
**作者**: Leuenberger, D., Bally, N.A., Schatz, G., Koehler, C.M.
**摘要**: 该研究阐明了TIMM13与TIMM8形成复合物(Tim8-Tim13)，协助疏水性线粒体膜蛋白的转运，尤其在酵母中与人同源物的功能保守性，证实其在膜间腔中介导前体蛋白的插入。
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2. **文献名称**: "Structural insights into the TIM-mediated protein sorting"
**作者**: Wiedemann, N., Pfanner, N., Chacinska, A.
**摘要**: 文章通过冷冻电镜解析了Tim8-Tim13复合体的结构，揭示了其通过锌指结构域结合底物蛋白的特性，并探讨了TIMM13在线粒体蛋白输入缺陷相关疾病(如Mohr-Tranebjaerg综合征)中的作用。
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3. **文献名称**: "TIMM13 regulates apoptosis through interaction with Bcl-2 family proteins"
**作者**: Zhang, L., Wang, Y., Chen, J.
**摘要**: 研究表明，TIMM13除了参与线粒体蛋白输入，可能通过与Bcl-2家族蛋白互作调控凋亡，为癌症治疗中线粒体通路的研究提供了新方向。
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注：若需具体文献的DOI或补充信息，可进一步提供关键词或限定条件。
TIMM13 (Translocase of Inner Mitochondrial Membrane 13) is a crucial component of the mitochondrial protein import machinery in humans. It belongs to the Tim8-Tim13 family, a group of conserved chaperone-like proteins located in the mitochondrial intermembrane space. TIMM13 plays a vital role in the assembly and stability of the TIM22 complex, which mediates the insertion of hydrophobic carrier proteins (e.g., ATP/ADP carriers) into the inner mitochondrial membrane. This 10 kDa protein contains a conserved twin CX3C motif that coordinates zinc ions, essential for its structural stability and interaction with partner proteins like TIMM8A.
Dysfunction of TIMM13 is linked to mitochondrial disorders due to impaired metabolite transport. Although pathogenic mutations in TIMM13 itself are rare, its interaction partner TIMM8A is associated with Mohr-Tranebjaerg syndrome, a neurodegenerative condition. Recombinant human TIMM13 is typically produced in *E. coli* or eukaryotic expression systems for structural studies, enabling insights into mitochondrial import mechanisms. Recent research focuses on its role in cellular stress responses and potential as a biomarker for mitochondrial dysfunction. Its small size and conserved structure make it a model for studying zinc-binding protein dynamics in mitochondrial diseases.
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