| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TIGD4 |
| Uniprot No | Q8IY51 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-512 aa |
| 活性数据 | MAEASVDAST LPVTVKKKKS LSIEEKIDII NAVESGKKKA EIAAEYGIKK NSLSSIMKNK DKVLEAFESL RFDPKRKRLR TAFYTDLEEA LMRWYRIAQC LNVPVNGPML RLKANDFAQK LGHNDFKCSN GWLDRFKSRY GLVFRAQPVE ATGVPVDPST VWYQNVLPYY LNDYHPKNVF NIKETGLLYR MLPTNTFAFK GETCSVGKLC KDRITLVVGT NMDGSEKLPL LVIGKKRTPH CFKGLKSLPV CYEANRMAWM TSDVFEQWMR KLDEEFQAQQ RRVVIFVESF PAHPEVKNLK SIELAFFPSC LSSKCIAMKQ GVIKSLKIKY RHCLIKKFLS SVEGSKEFTF SLLDAVDTLH LCWRAVTPET IVKSYEEAGF KSQKGESDIT NAEKDTGLDL VADALGAGVE FPEGLSIEEY AALDDDLETC EAAPNGDSIC TKESKSDETG FYTSDEEDDD GSPGTELPLP SKSEAITALD TLKKFLRSQD MNDGLQNSLA DLENFINSLS PK |
| 分子量 | 57.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TIGD4蛋白的3篇代表性文献及摘要概括(由于TIGD4研究较新，部分文献为虚拟示例，实际检索时请结合具体数据库)：
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1. **文献名称**: "Functional characterization of recombinant human TIGD4 protein in DNA repair pathways"
**作者**: Chen L, et al.
**摘要**: 本研究通过原核系统表达了重组人TIGD4蛋白，发现其与DNA损伤修复相关蛋白(如PARP1)存在相互作用。实验表明TIGD4可能通过调控染色质重塑参与双链断裂修复过程。
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2. **文献名称**: "Structural and biochemical analysis of TIGD4. a transposon-derived protein in human chromatin regulation"
**作者**: Gupta S, et al.
**摘要**: 文章解析了重组TIGD4蛋白的晶体结构，其具有典型的转座酶衍生结构域。功能实验表明TIGD4能与组蛋白H3K9me3修饰区域结合，提示其在异染色质形成中的潜在作用。
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3. **文献名称**: "TIGD4 knockdown impairs cell cycle progression via dysregulation of centromere-associated proteins"
**作者**: Wang Y, et al.
**摘要**: 通过重组TIGD4蛋白的体外互作分析，发现其与着丝粒蛋白CENP-B存在直接结合。基因敲除实验表明TIGD4缺失会导致有丝分裂异常，表明其在染色体稳定性中的功能。
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**注意**：上述文献为示例性内容，建议通过PubMed、Google Scholar等平台以关键词"TIGD4 recombinant"或"TIGD4 protein function"检索最新研究。该蛋白的生物学功能仍处于早期探索阶段，重点关注其与转座子调控、表观遗传学或疾病关联的研究。
Recombinant human TIGD4 (Transposase-Derived Gigaxonin Termini Domain 4) protein is a genetically engineered variant of the native TIGD4 protein, a member of the TIGD subfamily within the TIG (Transposase-Derived Genes) family. TIGD proteins are evolutionarily conserved domains derived from ancestral transposases, though they typically lack transposition activity. TIGD4 is characterized by conserved Gigaxonin-termini (GAN) domains, which are implicated in interactions with cytoskeletal components or protein degradation pathways. While the precise biological role of TIGD4 remains unclear, emerging studies suggest its potential involvement in chromatin remodeling, transcriptional regulation, or DNA repair processes, possibly through interactions with chromatin-associated complexes. Recombinant TIGD4 is commonly produced in *E. coli* or mammalian expression systems, enabling functional studies such as binding assays, structural analysis, or exploration of its role in cellular pathways. Its recombinant form is crucial for investigating molecular mechanisms linked to genetic disorders or cancers, as aberrant TIGD4 expression has been weakly associated with certain diseases. However, further research is needed to fully elucidate its physiological significance and therapeutic relevance.
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