| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TIA1 |
| Uniprot No | P31483 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-386 aa |
| 活性数据 | MEDEMPKTLYVGNLSRDVTEALILQLFSQIGPCKNCKMIMDTAGNDPYCFVEFHEHRHAAAALAAMNGRKIMGKEVKVNWATTPSSQKKDTSSSTVVSTQRSQDHFHVFVGDLSPEITTEDIKAAFAPFGRISDARVVKDMATGKSKGYGFVSFFNKWDAENAIQQMGGQWLGGRQIRTNWATRKPPAPKSTYESNTKQLSYDEVVNQSSPSNCTVYCGGVTSGLTEQLMRQTFSPFGQIMEIRVFPDKGYSFVRFNSHESAAHAIVSVNGTTIEGHVVKCYWGKETLDMINPVQQQNQIGYPQPYGQWGQWYGNAQQIGQYMPNGWQVPAYGMYGQAWNQQGFNQTQSSAPWMGPNYGVQPPQGQNGSMLPNQPSGYRVAGYETQ |
| 分子量 | 44.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TIA1蛋白的文献示例(虚构内容，仅作格式参考)：
1. **标题**：*Structural Insights into Recombinant Human TIA1 Protein in Stress Granule Assembly*
**作者**：Anderson, P., Kedersha, N.
**摘要**：研究通过重组表达的TIA1蛋白解析其RNA结合结构域的结构，揭示其在应激颗粒形成中通过液-液相分离调控mRNA翻译抑制的分子机制。
2. **标题**：*Recombinant TIA1 Expression and Its Role in Neurodegeneration*
**作者**：Li, S., et al.
**摘要**：报道了一种高效重组表达TIA1蛋白的方法，并验证其通过与tau蛋白互作抑制异常聚集的病理过程，提示其在阿尔茨海默病中的潜在保护作用。
3. **标题**：*TIA1 Phase Separation Dynamics Modulated by Post-Translational Modifications*
**作者**：Wang, Y., et al.
**摘要**：探讨磷酸化修饰对重组TIA1蛋白相变能力的影响，证明特定位点修饰可增强其应激颗粒组装功能并影响细胞应激响应。
4. **标题**：*Functional Characterization of Recombinant TIA1 in Apoptosis Regulation*
**作者**：García-Fernández, M., et al.
**摘要**：通过体外实验验证重组TIA1蛋白通过caspase通路调控细胞凋亡的功能，揭示其在癌症细胞死亡中的双刃剑作用。
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**注**：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台以关键词“recombinant TIA1 protein”“TIA1 function”检索，并结合近年研究筛选。
TIA1 (T-cell intracellular antigen 1) is a multifunctional RNA-binding protein involved in regulating gene expression at transcriptional and post-transcriptional levels. It belongs to the RNA-recognition motif (RRM) family, containing three RRM domains that mediate interactions with RNA/DNA, and a prion-like C-terminal domain enriched in glutamine and asparagine residues. This domain facilitates reversible liquid-liquid phase separation, enabling TIA1 to nucleate stress granules (SGs) – dynamic cytoplasmic condensates formed during cellular stress to sequester untranslated mRNAs and modulate translation.
Physiologically, TIA1 regulates mRNA splicing, stability, and translation, particularly under stress conditions. It promotes exon skipping by binding to uridine-rich sequences near splice sites and inhibits translation initiation by recruiting downstream effectors. TIA1 also participates in apoptosis via interactions with death receptor signaling pathways.
Dysregulation of TIA1 is implicated in neurodegenerative diseases (e.g., ALS, Alzheimer’s) and myodegenerative disorders. Pathogenic mutations in its prion-like domain may alter phase transition properties, leading to persistent SG formation and pathological protein aggregation.
Recombinant human TIA1 protein, often expressed in *E. coli* or mammalian systems with tags (e.g., GST, His), is widely used to study RNA-protein interactions, SG dynamics, and disease mechanisms *in vitro*. Its modular structure and phase-separating properties make it a model protein for investigating biomolecular condensates and proteostasis networks.
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