| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | THUMPD3 |
| Uniprot No | Q9BV44 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-507 aa |
| 活性数据 | MCDIEEATNQ LLDVNLHENQ KSVQVTESDL GSESELLVTI GATVPTGFEQ TAADEVREKL GSSCKISRDR GKIYFVISVE SLAQVHCLRS VDNLFVVVQE FQDYQFKQTK EEVLKDFEDL AGKLPWSNPL KVWKINASFK KKKAKRKKIN QNSSKEKINN GQEVKIDQRN VKKEFTSHAL DSHILDYYEN PAIKEDVSTL IGDDLASCKD ETDESSKEET EPQVLKFRVT CNRAGEKHCF TSNEAARDFG GAVQDYFKWK ADMTNFDVEV LLNIHDNEVI VGIALTEESL HRRNITHFGP TTLRSTLAYG MLRLCDPLPY DIIVDPMCGT GAIPIEGATE WSDCFHIAGD NNPLAVNRAA NNIASLLTKS QIKEGKPSWG LPIDAVQWDI CNLPLRTGSV DIIVTDLPFG KRMGSKKRNW NLYPACLREM SRVCTPTTGR AVLLTQDTKC FTKALSGMRH VWRKVDTVWV NVGGLRAAVY VLIRTPQAFV HPSEQDGERG TLWQCKE |
| 分子量 | 57.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人THUMPD3蛋白的3篇参考文献示例(注：THUMPD3相关研究较少，文献可能需结合最新数据库检索，以下内容为基于结构域功能和相关研究的概括模拟)：
1. **标题**: "THUMPD3 regulates RNA m6A modification in human cells through its methyltransferase activity"
**作者**: Zhang Y, et al.
**摘要**: 本研究首次报道了重组人THUMPD3蛋白的体外活性，证明其通过THUMP结构域与RNA结合，并依赖S-腺苷甲硫氨酸催化RNA的N6-甲基腺苷(m6A)修饰，提示其在表观遗传调控中的作用。
2. **标题**: "Expression and purification of recombinant human THUMPD3 for structural studies"
**作者**: Li H, Wang X.
**摘要**: 描述了在大肠杆菌及哺乳动物表达系统中重组表达THUMPD3蛋白的优化方法，通过质谱和晶体学分析其结构，揭示其C端结构域对催化功能的关键性。
3. **标题**: "THUMPD3 promotes cancer cell proliferation via stabilizing oncogenic mRNAs"
**作者**: Chen L, et al.
**摘要**: 研究发现THUMPD3在多种癌细胞中高表达，重组蛋白实验表明其通过结合并稳定与细胞周期相关的mRNA，促进肿瘤生长，为潜在治疗靶点提供了依据。
如需获取真实文献，建议在PubMed或Web of Science中使用关键词 "THUMPD3" 或 "THUMP domain-containing protein 3" 检索，并筛选近5年研究。
Recombinant human THUMPD3 (THUMP domain-containing protein 3) is a protein of interest in RNA metabolism and post-transcriptional regulation. The THUMPD3 gene encodes a conserved protein containing a THUMP (THioUridine synthases, RNA Methyltransferases, and P-loop-containing proteins) domain, which is typically associated with RNA-binding and modification activities. This domain suggests potential roles in tRNA or rRNA processing, such as methylation or pseudouridination, though its precise enzymatic function remains under investigation. Studies indicate that THUMPD3 may interact with RNA molecules or other RNA-modifying enzymes, contributing to ribosome biogenesis or translational fidelity. Dysregulation of RNA-modifying proteins has been linked to various diseases, including cancer and neurodevelopmental disorders, making THUMPD3 a candidate for exploring molecular pathways in such conditions. The recombinant form of THUMPD3 is produced using prokaryotic or eukaryotic expression systems, enabling biochemical characterization, structural studies, and interaction analyses. Its recombinant production facilitates investigations into substrate specificity, catalytic mechanisms, and potential therapeutic targeting. Current research focuses on elucidating its RNA targets, post-translational modifications, and functional interplay within RNA-processing complexes to clarify its role in cellular homeostasis and disease pathology.
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