| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEPT12 |
| Uniprot No | Q8IYM1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-358aa |
| 氨基酸序列 | MDPLRRSPSP CLSSQPSSPS TPPCEMLGPV GIEAVLDQLK IKAMKMGFEF NIMVVGQSGL GKSTMVNTLF KSKVWKSNPP GLGVPTPQTL QLHSLTHVIE EKGVKLKLTV TDTPGFGDQI NNDNCWDPIL GYINEQYEQY LQEEILITRQ RHIPDTRVHC CVYFVPPTGH CLRPLDIEFL QRLCRTVNVV PVIARADSLT MEEREAFRRR IQQNLRTHCI DVYPQMCFDE DINDKILNSK LRDRIPFAVV GADQEHLVNG RCVLGRKTKW GIIEVENMAH CEFPLLRDLL IRSHLQDLKD ITHNIHYENY RVIRLNESHL LPRGPGWVNL APASPGQLTT PRTFKVCRGA HDDSDDEF |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Characterization of SEPT12 recombination protein and its interaction with SEPT6 in human testes"**
- Authors: Lin YH, et al.
- 摘要：研究报道了重组SEPT12蛋白在原核系统中的表达与纯化，并证实其与SEPT6的相互作用，提示其在精子发生过程中可能参与调控细胞骨架结构。
2. **"Functional analysis of a novel SEPT12 mutation linked to male infertility using recombinant protein models"**
- Authors: Kuo YC, et al.
- 摘要：通过构建重组SEPT12突变体蛋白，发现其异常聚合能力可能导致精子形成缺陷，为男性不育的分子机制提供了实验依据。
3. **"Expression and purification of recombinant human SEPT12 for structural studies"**
- Authors: Hall PA, et al.
- 摘要：优化了SEPT12重组蛋白在大肠杆菌中的可溶性表达方案，并通过色谱技术获得高纯度蛋白，为后续结构解析(如X射线晶体学)奠定了基础。
4. **"SEPT12重组蛋白在体外自组装特性及其与微管结合的功能研究"**
- Authors: 张伟等.
- 摘要：利用重组SEPT12蛋白验证其在体外的自组装能力，并发现其与微管蛋白协同作用，可能影响精子尾部形成过程中的细胞动力学。
注：以上文献信息为示例，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。
**Background of SEPT12 Recombinant Protein**
SEPT12 (Septin 12) is a member of the septin family, a group of GTP-binding proteins involved in diverse cellular processes, including cytokinesis, cytoskeletal organization, vesicle trafficking, and cell polarity. Septins form heteropolymeric complexes that act as scaffolds or diffusion barriers, contributing to membrane remodeling and signaling. SEPT12 is specifically expressed in germ cells and plays a critical role in spermatogenesis. Studies highlight its importance in sperm head shaping, tail formation, and acrosome reaction during male gamete development. Mutations or dysregulation of SEPT12 are linked to male infertility, teratozoospermia (abnormal sperm morphology), and recurrent pregnancy loss, underscoring its essential role in reproductive health.
The recombinant SEPT12 protein is engineered for *in vitro* studies to elucidate its molecular functions and interactions. Typically produced in bacterial (e.g., *E. coli*) or mammalian expression systems, the recombinant protein retains functional domains, including the GTPase domain and coiled-coil regions, enabling investigations into its structure, polymerization, and binding partners (e.g., other septins, cytoskeletal components). Researchers utilize SEPT12 recombinant protein to study its role in spermatogenesis defects, model pathogenic mutations, and screen for therapeutic targets in infertility. Additionally, it serves as an antigen for antibody development in diagnostic assays.
Beyond reproduction, SEPT12 has implications in cancer and neurodegenerative diseases, as septins are dysregulated in tumorigenesis and neuronal degeneration. The recombinant protein thus provides a versatile tool for dissecting septin biology across multiple disciplines, bridging molecular mechanisms to clinical applications.
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