关键信息
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基因名
CCDC9
- 应用
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别名
CCDC9Coiled-coil domain-containing Protein 9
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9Y3X0
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表达区间
1-531aa
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氨基酸序列
MAATLDLKSK EEKDAELDKR IEALRRKNEA LIRRYQEIEE DRKKAELEGV AVTAPRKGRS VEKENVAVES EKNLGPSRRS PGTPRPPGAS KGGRTPPQQG GRAGMGRASR SWEGSPGEQP RGGGAGGRGR RGRGRGSPHL SGAGDTSISD RKSKEWEERR RQNIEKMNEE MEKIAEYERN QREGVLEPNP VRNFLDDPRR RSGPLEESER DRREESRRHG RNWGGPDFER VRCGLEHERQ GRRAGLGSAG DMTLSMTGRE RSEYLRWKQE REKIDQERLQ RHRKPTGQWR REWDAEKTDG MFKDGPVPAH EPSHRYDDQA WARPPKPPTF GEFLSQHKAE ASSRRRRKSS RPQAKAAPRA YSDHDDRWET KEGAASPAPE TPQPTSPETS PKETPMQPPE IPAPAHRPPE DEGEENEGEE DEEWEDISED EEEEEIEVEE GDEEEPAQDH QAPEAAPTGI PCSEQAHGVP FSPEEPLLEP QAPGTPSSPF SPPSGHQPVS DWGEEVELNS PRTTHLAGAL SPGEAWPFES V
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分子量
86.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
CCDC9 (Coiled-Coil Domain Containing 9) is a gene that encodes a protein implicated in various cellular processes, including DNA repair, stress response, and transcription regulation. Its role in maintaining genomic integrity makes it a critical subject of study in the context of cancer biology, as mutations or alterations in DNA repair pathways can lead to increased susceptibility to tumorigenesis. Research has shown that CCDC9 interacts with several key proteins involved in the DNA repair machinery, suggesting its involvement in cellular responses to DNA damage. Furthermore, alterations in CCDC9 expression levels have been correlated with poor prognosis in certain cancers, highlighting its potential as a biomarker for disease progression. The production of recombinant CCDC9 protein is essential for functional studies aimed at elucidating its molecular mechanisms and interactions within the cell. By using techniques such as molecular cloning and expression in suitable host cells, researchers can generate large quantities of this protein for biochemical assays and structural analysis. Understanding the role of CCDC9 in cellular processes not only sheds light on fundamental biological mechanisms but also opens avenues for the development of targeted therapies in cancer treatment and other diseases where DNA damage plays a pivotal role.
