关键信息
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基因名
RNF148
- 应用
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别名
RNF148; RING finger protein 148
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8N7C7
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表达区间
1-305 aa
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氨基酸序列
MSFLRITPSTHSSVSSGLLRLSIFLLLSFPDSNGKAIWTAHLNITFQVGNEITSELGESGVFGNHSPLERVSGVVALPEGWNQNACHPLTNFSRPKQADSWLALIERGGCTFTHKINVAAEKGANGVIIYNYQGTGSKVFPMSHQGTENIVAVMISNLKGMEILHSIQKGVYVTVIIEVGRMHMQWVSHYIMYLFTFLAATIAYFYLDCVWRLTPRVPNSFTRRRSQIKTDVKKAIDQLQLRVLKEGDEELDLNEDNCVVCFDTYKPQDVVRILTCKHFFHKACIDPWLLAHRTCPMCKCDILKT
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分子量
60.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
RNF148 is an E3 ubiquitin ligase that plays a critical role in the regulation of protein degradation and signal transduction pathways, making it essential for various cellular processes, including DNA damage response, immune signaling, and cell proliferation. Recent studies have shown that RNF148 might be involved in modifying the stability of key proteins linked to cancer progression and other diseases. Its ability to regulate the ubiquitination process has drawn significant attention in the field of molecular biology and therapeutic research, as targeting RNF148 could provide novel strategies for cancer treatment and enhancement of immune responses. The characterization of recombinant RNF148 protein is crucial for understanding its ubiquitin ligase activity, substrate specificity, and structural properties. By producing this protein in a recombinant form, researchers aim to elucidate its functional mechanisms, interactions with various substrates, and its potential as a therapeutical target. This research is not only pivotal for uncovering the biological roles of RNF148 but also for developing drugs that could modulate its activity in pathological conditions. Therefore, the study of RNF148 recombinant protein is a promising avenue for advancing our knowledge of cellular regulation and for paving the way towards innovative treatment options in oncology and immunotherapy.
