| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC27A1 |
| Uniprot No | Q6PCB7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-646 aa |
| 活性数据 | MRAPGAGAASVVSLALLWLLGLPWTWSAAAALGVYVGSGGWRFLRIVCKTARRDLFGLSVLIRVRLELRRHQRAGHTIPRIFQAVVQRQPERLALVDAGTGECWTFAQLDAYSNAVANLFRQLGFAPGDVVAIFLEGRPEFVGLWLGLAKAGMEAALLNVNLRREPLAFCLGTSGAKALIFGGEMVAAVAEVSGHLGKSLIKFCSGDLGPEGILPDTHLLDPLLKEASTAPLAQIPSKGMDDRLFYIYTSGTTGLPKAAIVVHSRYYRMAAFGHHAYRMQAADVLYDCLPLYHSAGNIIGVGQCLIYGLTVVLRKKFSASRFWDDCIKYNCTVVQYIGEICRYLLKQPVREAERRHRVRLAVGNGLRPAIWEEFTERFGVRQIGEFYGATECNCSIANMDGKVGSCGFNSRILPHVYPIRLVKVNEDTMELLRDAQGLCIPCQAGEPGLLVGQINQQDPLRRFDGYVSESATSKKIAHSVFSKGDSAYLSGDVLVMDELGYMYFRDRSGDTFRWRGENVSTTEVEGVLSRLLGQTDVAVYGVAVPGVEGKAGMAAVADPHSLLDPNAIYQELQKVLAPYARPIFLRLLPQVDTTGTFKIQKTRLQREGFDPRQTSDRLFFLDLKQGHYLPLNEAVYTRICSGAFAL |
| 分子量 | 97.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC27A1(FATP1)蛋白的3篇代表性文献，按研究内容分类：
1. **文献名称**：**"The fatty acid transport protein (FATP1) is a very long chain acyl-CoA synthetase"**
**作者**：Jia Z, Pei Z, Maiguel D, Toomer CJ, Watkins PA
**摘要**：通过重组表达人SLC27A1蛋白，揭示了其作为超长链脂肪酸(VLCFA)酰基辅酶A合成酶的活性，表明FATP1不仅参与脂肪酸转运，还直接催化脂肪酸代谢的第一步反应。
2. **文献名称**：**"Functional characterization of fatty acid transport protein 1 in a human intestinal cell line"**
**作者**：Zou Z, Zeng F, Xu W, Wang C et al.
**摘要**：在Caco-2肠道细胞中重组过表达SLC27A1.发现其显著增强细胞对长链脂肪酸的摄取能力，并通过胰岛素调控分析，证实其在脂质吸收中的关键作用。
3. **文献名称**：**"Recombinant human FATP1 synthesis and characterization: A model for ligand binding studies"**
**作者**：Milger K, Herrmann T, Becker C et al.
**摘要**：报道在大肠杆菌系统中高效表达并纯化重组人SLC27A1蛋白，结合荧光标记技术分析其与脂肪酸配体的结合特性，为结构功能研究提供基础。
4. **文献名称**：**"FATP1 is an insulin-sensitive fatty acid transporter involved in diet-induced obesity"**
**作者**：Wu Q, Ortegon J, Sheth B et al.
**摘要**：利用重组SLC27A1蛋白及基因敲除小鼠模型，证实该蛋白在胰岛素敏感性脂肪酸摄取中的作用，并揭示其在饮食诱导肥胖中的代谢调控机制。
这些文献涵盖了重组SLC27A1蛋白的功能验证、代谢机制、配体互作及疾病关联研究，可作为相关领域的参考依据。
Solute carrier family 27 member 1 (SLC27A1), also known as fatty acid transport protein 1 (FATP1), is a transmembrane protein encoded by the *SLC27A1* gene in humans. It belongs to the SLC27 family of fatty acid transporters and plays a critical role in cellular long-chain fatty acid uptake and metabolism. SLC27A1 is predominantly expressed in tissues with high lipid turnover, including adipose tissue, skeletal muscle, liver, and heart. Structurally, it contains an N-terminal transmembrane domain and a C-terminal adenosine monophosphate (AMP)-binding domain, which facilitates its enzymatic activity as an acyl-CoA synthetase. This dual functionality allows SLC27A1 to not only transport fatty acids across membranes but also activate them to acyl-CoA esters, a crucial step for lipid storage, membrane synthesis, and energy production.
Studies have linked SLC27A1 to metabolic disorders such as obesity, insulin resistance, and type 2 diabetes. In adipocytes, it regulates fatty acid influx and triglyceride synthesis, while in muscle cells, it influences fatty acid oxidation and energy expenditure. Dysregulation of SLC27A1 has been implicated in altered lipid partitioning and systemic metabolic homeostasis. Recombinant human SLC27A1 protein, produced via heterologous expression systems like *E. coli* or mammalian cells, is widely used to study its structure-function relationships, substrate specificity, and interaction with pharmacological inhibitors. Its role as a potential therapeutic target for metabolic syndromes and cancer (due to altered lipid metabolism in tumors) continues to drive research interest.
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