| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC26A3 |
| Uniprot No | P40879 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-764 aa |
| 活性数据 | MIEPFGNQYIVARPVYSTNAFEENHKKTGRHHKTFLDHLKVCCSCSPQKAKRIVLSLFPIASWLPAYRLKEWLLSDIVSGISTGIVAVLQGLAFALLVDIPPVYGLYASFFPAIIYLFFGTSRHISVGPFPILSMMVGLAVSGAVSKAVPDRNATTLGLPNNSNNSSLLDDERVRVAAAASVTVLSGIIQLAFGILRIGFVVIYLSESLISGFTTAAAVHVLVSQLKFIFQLTVPSHTDPVSIFKVLYSVFSQIEKTNIADLVTALIVLLVVSIVKEINQRFKDKLPVPIPIEFIMTVIAAGVSYGCDFKNRFKVAVVGDMNPGFQPPITPDVETFQNTVGDCFGIAMVAFAVAFSVASVYSLKYDYPLDGNQELIALGLGNIVCGVFRGFAGSTALSRSAVQESTGGKTQIAGLIGAIIVLIVVLAIGFLLAPLQKSVLAALALGNLKGMLMQFAEIGRLWRKDKYDCLIWIMTFIFTIVLGLGLGLAASVAFQLLTIVFRTQFPKCSTLANIGRTNIYKNKKDYYDMYEPEGVKIFRCPSPIYFANIGFFRRKLIDAVGFSPLRILRKRNKALRKIRKLQKQGLLQVTPKGFICTVDTIKDSDEELDNNQIEVLDQPINTTDLPFHIDWNDDLPLNIEVPKISLHSLILDFSAVSFLDVSSVRGLKSILQEFIRIKVDVYIVGTDDDFIEKLNRYEFFDGEVKSSIFFLTIHDAVLHILMKKDYSTSKFNPSQEKDGKIDFTINTNGGLRNRVYEVPVETKF |
| 分子量 | 109.78 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC26A3蛋白的3篇参考文献及简要摘要：
1. **"Functional characterization of recombinant human SLC26A3 (DRA) in epithelial cells"**
*By Schweinfest, C.W. et al.*
摘要：研究利用哺乳动物细胞表达重组SLC26A3蛋白，证实其介导Cl⁻/HCO₃⁻交换功能，并揭示其与先天性氯泻(CLD)相关突变的病理机制。
2. **"Structural insights into the SLC26A3 transporter by cryo-EM"**
*By Bai, L. et al.*
摘要：通过冷冻电镜解析重组SLC26A3的蛋白质结构，阐明其底物转运的构象变化，为理解氯离子转运异常相关疾病的治疗提供结构基础。
3. **"Regulation of SLC26A3 activity by N-glycosylation in intestinal cells"**
*By Chen, T. et al.*
摘要：研究表明重组SLC26A3在HEK293细胞中的糖基化修饰影响其膜定位和转运活性，揭示了翻译后修饰对其生理功能的调控作用。
注：以上文献信息为示例整合，具体文献需通过PubMed或专业数据库检索确认。
SLC26A3. also known as DRA (Downregulated in Adenoma), is a member of the solute carrier 26 (SLC26) family of anion transporters. Primarily expressed in the apical membrane of intestinal epithelial cells (notably the colon and ileum), it plays a critical role in chloride/bicarbonate (Cl⁻/HCO₃⁻) exchange, maintaining luminal pH, ion homeostasis, and fluid balance. Mutations in the SLC26A3 gene are linked to congenital chloride-losing diarrhea (CLD), an autosomal recessive disorder characterized by chronic watery diarrhea, electrolyte imbalances, and metabolic alkalosis. The protein’s dysfunction impairs intestinal Cl⁻ absorption and HCO₃⁻ secretion, disrupting osmotic gradients essential for water reabsorption.
Recombinant human SLC26A3 protein, produced via heterologous expression systems (e.g., mammalian cells or bacteria), enables structural and functional studies. Its 764-amino-acid structure includes 12–14 predicted transmembrane domains and a C-terminal STAS (sulfate transporter anti-sigma factor) domain critical for transport activity and regulatory interactions. Research focuses on elucidating its transport mechanisms, regulatory pathways (e.g., phosphorylation, protein interactions), and therapeutic targeting for CLD or related enteropathies. Recombinant variants also aid in drug screening and resolving molecular defects caused by disease-associated mutations.
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