| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC26A1 |
| Uniprot No | Q9H2B4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 0 |
| 活性数据 | MDESPEPLQQGRGPVPVRRQRPAPRGLREMLKARLWCSCSCSVLCVRALVQDLLPATRWLRQYRPREYLAGDVMSGLVIGIILVPQAIAYSLLAGLQPIYSLYTSFFANLIYFLMGTSRHVSVGIFSLLCLMVGQVVDRELQLAGFDPSQDGLQPGANSSTLNGSAAMLDCGRDCYAIRVATALTLMTGLYQVLMGVLRLGFVSAYLSQPLLDGFAMGASVTILTSQLKHLLGVRIPRHQGPGMVVLTWLSLLRGAGQANVCDVVTSTVCLAVLLAAKELSDRYRHRLRVPLPTELLVIVVATLVSHFGQLHKRFGSSVAGDIPTGFMPPQVPEPRLMQRVALDAVALALVAAAFSISLAEMFARSHGYSVRANQELLAVGCCNVLPAFLHCFATSAALAKSLVKTATGCRTQLSSVVSATVVLLVLLALAPLFHDLQRSVLACVIVVSLRGALRKVWDLPRLWRMSPADALVWAGTAATCMLVSTEAGLLAGVILSLLSLAGRTQRPRTALLARIGDTAFYEDATEFEGLVPEPGVRVFRFGGPLYYANKDFFLQSLYSLTGLDAGCMAARRKEGGSETGVGEGGPAQGEDLGPVSTRAALVPAAAGFHTVVIDCAPLLFLDAAGVSTLQDLRRDYGALGISLLLACCSPPVRDILSRGGFLGEGPGDTAEEEQLFLSVHDAVQTARARHRELEATDAHL |
| 分子量 | 77.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC26A1蛋白的3篇代表性文献及其摘要概括：
1. **《SLC26A1 is a major transporter of sulfate in the renal proximal tubule》**
- **作者**: Dawson PA et al. (2021)
- **摘要**: 本研究通过重组人SLC26A1蛋白的体外功能分析，结合基因敲除小鼠模型，证实SLC26A1是肾脏近端小管上皮细胞中硫酸盐/草酸转运的关键蛋白，其缺失导致血浆硫酸盐水平升高和草酸代谢紊乱，可能参与肾结石的发病机制。
2. **《Structural insights into the human SLC26A1 mediated anion transport mechanism》**
- **作者**: Li Q et al. (2019)
- **摘要**: 利用冷冻电镜技术解析了重组表达的人源SLC26A1蛋白结构，揭示了其跨膜结构域和STAS结构域的协同作用，阐明了其对硫酸盐、草酸等二价阴离子的选择性转运机制，为靶向药物设计提供了结构基础。
3. **《Hepatic SLC26A1 deficiency induces mitochondrial dysfunction and promotes liver injury》**
- **作者**: Liu Y et al. (2022)
- **摘要**: 通过肝细胞特异性敲除SLC26A1的小鼠实验，发现该蛋白在肝细胞线粒体硫代谢中起重要作用，其缺失导致谷胱甘肽合成障碍和氧化应激，显著加重药物性肝损伤，提示SLC26A1是肝脏解毒功能的关键调控因子。
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注：以上文献信息为示例性内容，实际引用时请核对原始文献准确性。若需具体文章DOI或补充更多研究，建议在PubMed等平台以"SLC26A1 recombinant"或"SLC26A1 anion transport"为关键词检索。
The solute carrier family 26 member 1 (SLC26A1), also known as sulfate anion transporter 1 (SAT1), is a transmembrane protein encoded by the SLC26A1 gene in humans. It belongs to the SLC26 family of anion transporters, which facilitate the movement of various substrates including sulfate, oxalate, bicarbonate, and chloride across cellular membranes. Structurally, SLC26A1 contains 12 predicted transmembrane domains and a conserved STAS (sulfate transporter and antisigma factor antagonist) domain critical for its transport activity and regulatory functions.
Primarily expressed in the liver, kidneys, and gastrointestinal tract, SLC26A1 plays a vital role in sulfate homeostasis and detoxification processes. In hepatocytes, it mediates sulfate efflux at the basolateral membrane, balancing intracellular sulfate levels essential for phase II metabolism. Pathologically, SLC26A1 dysfunction has been linked to hyperoxaluria, hepatobiliary disorders, and drug-induced liver injury due to impaired sulfate-dependent detoxification.
Recombinant human SLC26A1 protein is engineered for functional studies, enabling researchers to investigate its transport kinetics, substrate specificity, and interactions with therapeutic agents. Produced via heterologous expression systems (e.g., mammalian or insect cells), the purified protein retains post-translational modifications necessary for physiological activity. This tool supports drug discovery targeting anion transport-related diseases and mechanistic studies of SLC26A1's role in cellular metabolism and disease pathogenesis.
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