| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC25A48 |
| Uniprot No | Q6ZT89 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-157 aa |
| 活性数据 | MGSFQLEDFAAGWIGGAASVIVGHPLDTVKTRLQAGVGYGNTLSCIRVVYRRESMFGFFKGMSFPLASIAVYNSVVFGVFSNTQRFLSQHRCGEPEASPPRTLSDLLLASMVAGVVSVGLGGPVDLIKIRLQMQTQPFRDGGEHRMTTVFPGPHLCM |
| 分子量 | 43.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC25A48蛋白的假设性参考文献(基于相关领域研究推测，具体文献需进一步验证)：
1. **文献名称**: *"Functional characterization of the human mitochondrial carrier SLC25A48 in amino acid transport"*
**作者**: Zhang L, et al.
**摘要**: 本研究通过重组表达SLC25A48蛋白于酵母突变体，证实其作为线粒体中性氨基酸转运体的功能，并揭示其在细胞代谢中的潜在作用。
2. **文献名称**: *"Structural insights into the SLC25A48 transporter by cryo-EM analysis"*
**作者**: Wang Y, et al.
**摘要**: 利用冷冻电镜解析重组人SLC25A48蛋白的三维结构，发现其具有典型线粒体载体蛋白的折叠模式，并提出底物结合的关键位点。
3. **文献名称**: *"SLC25A48 regulates one-carbon metabolism via mitochondrial folate transport"*
**作者**: Kim H, et al.
**摘要**: 通过HeLa细胞中重组SLC25A48的敲除/回补实验，证明其参与一碳单位代谢，可能通过转运叶酸衍生物影响核苷酸合成。
4. **文献名称**: *"SLC25A48 overexpression exacerbates oxidative stress in metabolic disorders"*
**作者**: Rossi M, et al.
**摘要**: 在大鼠模型中过表达重组人SLC25A48蛋白，发现其通过干扰线粒体氧化还原平衡，与胰岛素抵抗相关代谢疾病存在关联。
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**注**：以上文献为示例性内容，实际研究需以真实发表的论文为准。建议通过PubMed或Web of Science以**“SLC25A48”**或**“mitochondrial carrier protein 48”**为关键词检索最新文献。
**Background of Recombinant Human SLC25A48 Protein**
The SLC25A48 protein, part of the SLC25 mitochondrial carrier family, facilitates the transport of metabolites across mitochondrial membranes, crucial for cellular energy metabolism. This nuclear-encoded protein localizes to mitochondria and is implicated in shuttling small molecules like vitamins, nucleotides, or cofactors, though its precise substrate specificity remains under investigation. Recent studies suggest SLC25A48 may interact with thiamine derivatives, potentially influencing mitochondrial thiamine pyrophosphate (TPP) homeostasis, which is vital for carbohydrate metabolism and energy production.
Dysregulation of SLC25A48 has been linked to metabolic disorders and mitochondrial dysfunction. For instance, altered expression is observed in conditions like diabetes and neurodegenerative diseases, highlighting its role in metabolic and oxidative stress pathways. Recombinant human SLC25A48 is engineered for research to study its structure-function relationships, substrate profiling, and regulatory mechanisms. Its production typically involves heterologous expression systems (e.g., E. coli or mammalian cells) followed by purification, enabling in vitro assays or therapeutic exploration.
Current research focuses on its potential as a therapeutic target, particularly in metabolic syndromes or mitochondrial diseases. However, gaps persist in understanding its physiological partners, transport kinetics, and tissue-specific roles, warranting further investigation.
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