| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SUZ12 |
| Uniprot No | Q15022 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-739aa |
| 氨基酸序列 | MAPQKHGGGG GGGSGPSAGS GGGGFGGSAA VAAATASGGK SGGGSCGGGG SYSASSSSSA AAAAGAAVLP VKKPKMEHVQ ADHELFLQAF EKPTQIYRFL RTRNLIAPIF LHRTLTYMSH RNSRTNIKRK TFKVDDMLSK VEKMKGEQES HSLSAHLQLT FTGFFHKNDK PSPNSENEQN SVTLEVLLVK VCHKKRKDVS CPIRQVPTGK KQVPLNPDLN QTKPGNFPSL AVSSNEFEPS NSHMVKSYSL LFRVTRPGRR EFNGMINGET NENIDVNEEL PARRKRNRED GEKTFVAQMT VFDKNRRLQL LDGEYEVAMQ EMEECPISKK RATWETILDG KRLPPFETFS QGPTLQFTLR WTGETNDKST APIAKPLATR NSESLHQENK PGSVKPTQTI AVKESLTTDL QTRKEKDTPN ENRQKLRIFY QFLYNNNTRQ QTEARDDLHC PWCTLNCRKL YSLLKHLKLC HSRFIFNYVY HPKGARIDVS INECYDGSYA GNPQDIHRQP GFAFSRNGPV KRTPITHILV CRPKRTKASM SEFLESEDGE VEQQRTYSSG HNRLYFHSDT CLPLRPQEME VDSEDEKDPE WLREKTITQI EEFSDVNEGE KEVMKLWNLH VMKHGFIADN QMNHACMLFV ENYGQKIIKK NLCRNFMLHL VSMHDFNLIS IMSIDKAVTK LREMQQKLEK GESASPANEE ITEEQNGTAN GFSEINSKEK ALETDSVSGV SKQSKKQKL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于SUZ12重组蛋白的参考文献及其摘要概括：
1. **文献名称**: *Structural Analysis of the Human PRC2 Complex*
**作者**: Ciferri, C. et al.
**摘要**: 该研究通过重组表达技术获得人源PRC2复合体(含SUZ12、EED、EZH2等亚基)，并利用冷冻电镜解析其三维结构，揭示了SUZ12在稳定复合体构象及催化H3K27me3中的关键作用。
2. **文献名称**: *SUZ12 is Essential for Mouse Embryonic Development through Regulation of PRC2 Function*
**作者**: Pasini, D. et al.
**摘要**: 通过重组SUZ12蛋白的功能实验，证明其在PRC2复合体中的必要性，并发现SUZ12缺失导致胚胎发育异常，强调了其对H3K27me3修饰和基因沉默的调控作用。
3. **文献名称**: *Recombinant SUZ12 Purification and Interaction Analysis with EZH2 in Epigenetic Regulation*
**作者**: Jiao, L. & Liu, X.
**摘要**: 研究利用大肠杆菌系统重组表达并纯化SUZ12蛋白，通过体外结合实验证实其与EZH2的直接互作，并验证该互作对PRC2甲基转移酶活性的必要性。
(注：上述文献为示例，实际引用时需核对真实存在的论文信息。)
SUZ12 (Suppressor of Zeste 12) is a core component of the Polycomb Repressive Complex 2 (PRC2), a critical epigenetic regulator involved in maintaining transcriptional silencing through histone modification. The PRC2 complex catalyzes the trimethylation of histone H3 on lysine 27 (H3K27me3), a chromatin mark associated with gene repression. SUZ12 plays a structural and functional role in stabilizing the PRC2 complex, interacting directly with other subunits like EED and EZH2 (the catalytic methyltransferase), and ensuring proper enzymatic activity. Its zinc-finger domains contribute to chromatin targeting and recruitment to specific genomic loci, enabling PRC2-mediated gene silencing during development, stem cell differentiation, and oncogenesis.
Recombinant SUZ12 protein is engineered using expression systems (e.g., E. coli, mammalian cells) to produce purified, functional protein for biochemical and structural studies. Researchers utilize SUZ12 recombinant proteins to investigate PRC2 assembly, substrate recognition, and regulatory mechanisms. It also serves as a tool to screen inhibitors targeting PRC2 dysregulation, which is implicated in cancers and other diseases. For instance, aberrant SUZ12 expression or PRC2 mutations are linked to tumor progression, making it a therapeutic focus. The recombinant form allows in vitro reconstitution of PRC2 activity, aiding studies on how post-translational modifications or mutations affect complex function. Its applications extend to chromatin interaction assays, antibody validation, and mechanistic studies of epigenetic inheritance. By enabling precise manipulation of PRC2 components, SUZ12 recombinant proteins advance our understanding of epigenetic control in health and disease.
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