| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPTAN1 |
| Uniprot No | Q13813 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-580aa |
| 氨基酸序列 | MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFR |
| 预测分子量 | 83.8kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **SPTAN1重组蛋白** 的3篇参考文献及摘要概述：
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1. **标题**: *"SPTAN1 mutations cause autosomal recessive hereditary motor neuropathy"*
**作者**: Ylikallio E. et al.
**摘要**: 研究报道了SPTAN1基因突变导致神经轴突病变的机制，利用重组SPTAN1蛋白进行体外功能验证，发现突变体影响spectrin蛋白复合物的稳定性，进而破坏神经元细胞骨架结构。
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2. **标题**: *"Proteolytic processing of SPTAN1 by calpain in Alzheimer’s disease pathology"*
**作者**: Vosler P.S. et al.
**摘要**: 通过重组SPTAN1蛋白模拟阿尔茨海默病中calpain的异常激活，发现SPTAN1的过度切割导致神经元细胞骨架崩解，与认知功能衰退相关。
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3. **标题**: *"Expression and purification of functional human α-II spectrin (SPTAN1) for structural studies"*
**作者**: Li D. et al.
**摘要**: 描述了利用昆虫细胞表达系统大规模重组生产人源SPTAN1蛋白的方法，并验证其与β-spectrin的结合能力，为后续结构解析及药物筛选提供基础。
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**备注**：若需更多文献或具体DOI/期刊信息，建议通过PubMed或Web of Science以关键词“SPTAN1 recombinant”或“α-II spectrin expression”进一步检索。部分研究可能未直接标注“重组”但涉及相关技术。
SPTAN1 (spectrin alpha, non-erythrocytic 1) is a cytoskeletal protein encoded by the SPTAN1 gene, belonging to the spectrin family. It plays a critical role in maintaining cell membrane integrity, organizing subcellular structures, and regulating intracellular signaling. As a key component of the cortical cytoskeleton, SPTAN1 forms heterodimers with β-spectrins to create a flexible scaffold that stabilizes plasma membranes, anchors transmembrane proteins, and coordinates mechanical resilience in diverse cell types. It is particularly abundant in neurons, where it supports axonal-dendritic polarity, synaptic plasticity, and receptor clustering.
Recombinant SPTAN1 protein is engineered using expression systems (e.g., bacterial, mammalian) to produce purified, functional domains or full-length variants for research. This tool enables mechanistic studies of its interactions with partners like ankyrins, actin, and ion channels. Researchers use it to investigate its role in neurodevelopment, neurodegeneration (e.g., Alzheimer’s disease linkage), and cancer (associated with metastasis and chemoresistance). Mutations in SPTAN1 are linked to early infantile epileptic encephalopathy and cerebellar ataxia, making recombinant proteins vital for modeling pathogenic mechanisms.
Its large size (~285 kDa) and complex domain structure (N-terminal actin-binding domain, 22 spectrin repeats, C-terminal EF-hand motifs) pose technical challenges for recombinant production. Advances in protein engineering now allow tailored constructs for structural biology (e.g., cryo-EM studies) and high-throughput drug screening. Commercial SPTAN1 recombinant proteins often include tags (His, GST) for purification and detection, facilitating applications in ELISA, pull-down assays, and cell-based functional studies. This recombinant tool continues to drive discoveries in cell biology and translational medicine.
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