| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SETD6 |
| Uniprot No | Q8TBK2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-473 aa |
| 活性数据 | MATQAKRPRV AGPVDGGDLD PVACFLSWCR RVGLELSPKV SERAGGRRTR GGARAALTSP PAQVAVSRQG TVAGYGMVAR ESVQAGELLF VVPRAALLSQ HTCSIGGLLE RERVALQSQS GWVPLLLALL HELQAPASRW RPYFALWPEL GRLEHPMFWP EEERRCLLQG TGVPEAVEKD LANIRSEYQS IVLPFMEAHP DLFSLRVRSL ELYHQLVALV MAYSFQEPLE EEEDEKEPNS PVMVPAADIL NHLANHNANL EYSANCLRMV ATQPIPKGHE IFNTYGQMAN WQLIHMYGFV EPYPDNTDDT ADIQMVTVRE AALQGTKTEA ERHLVYERWD FLCKLEMVGE EGAFVIGREE VLTEEELTTT LKVLCMPAEE FRELKDQDGG GDDKREEGSL TITNIPKLKA SWRQLLQNSV LLTLQTYATD LKTDQGLLSN KEVYAKLSWR EQQALQVRYG QKMILHQLLE LTS |
| 分子量 | 53.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SETD6蛋白的3篇示例参考文献(示例为虚构内容，供参考)：
1. **文献名称**: "SETD6-mediated methylation of histone H3K4 suppresses transcriptional activation in human cells"
**作者**: Lachmann A. et al.
**摘要**: 本研究揭示了SETD6作为组蛋白甲基转移酶，通过催化H3K4的单甲基化修饰抑制靶基因的转录活性，调控细胞分化过程。
2. **文献名称**: "SETD6 regulates NF-κB signaling via non-catalytic interaction with RelA"
**作者**: Levy D. et al.
**摘要**: 发现SETD6通过非酶依赖方式与转录因子RelA结合，促进NF-κB通路负反馈调节，影响炎症反应和细胞存活。
3. **文献名称**: "Structural basis of SETD6-dependent lysine methylation in Wnt/β-catenin pathway"
**作者**: Morett E. et al.
**摘要**: 通过晶体结构解析，阐明SETD6对β-catenin蛋白的甲基化修饰机制，证实其通过表观遗传调控影响Wnt通路活性与肿瘤发生。
---
*注：以上为模拟生成的参考文献示例，实际文献需通过PubMed、Google Scholar等平台检索确认。真实研究中SETD6可能涉及染色质修饰、信号通路调控及疾病关联等方向。*
SETD6 (SET Domain Containing 6) is a member of the SET domain-containing methyltransferase family, primarily involved in epigenetic regulation through lysine methylation of histones and non-histone proteins. It catalyzes the transfer of methyl groups to specific lysine residues, notably histone H2A at lysine 7 (H2AK7me) and non-histone substrates like the transcription factor NF-κB subunit RelA (p65) and AKT kinase. Structurally, SETD6 contains a catalytic SET domain at its C-terminus, essential for methyltransferase activity, and disordered N-terminal regions that may mediate protein interactions.
Functionally, SETD6 plays roles in transcriptional repression, chromatin remodeling, and cellular signaling pathways. It regulates processes such as inflammatory responses, stress signaling, and cell proliferation by modulating key pathways, including Wnt/β-catenin and NF-κB. Dysregulation of SETD6 has been implicated in diseases like cancer, metabolic disorders, and chronic inflammation, making it a potential therapeutic target.
Recombinant human SETD6 protein is widely used in biochemical studies to dissect its enzymatic mechanisms, substrate specificity, and interactions. In vitro assays using purified SETD6 have helped identify inhibitors and explore its role in epigenetic crosstalk. Despite progress, its full range of substrates and context-dependent regulatory functions remain areas of active research.
×
