NT-ProANP (N-terminal pro-atrial natriuretic peptide) is a biologically inactive fragment derived from the precursor protein proANP, which is primarily synthesized and secreted by cardiac atrial myocytes in response to ventricular stretch, volume overload, or pressure stress. ANP itself is a key hormone involved in regulating blood pressure, fluid balance, and cardiovascular homeostasis. During proANP processing, the mature active hormone ANP (C-terminal 28-amino-acid peptide) is cleaved and co-released with NT-ProANP (the N-terminal 1–98 amino acid segment), making NT-ProANP a stable biomarker for assessing cardiac function.
Recombinant NT-ProANP refers to the engineered protein produced via molecular cloning and expression systems (e.g., E. coli, mammalian cells) to mimic the native peptide’s structure. Its production enables standardized research and clinical applications, particularly in studying heart failure, hypertension, and renal dysfunction. Unlike ANP, NT-ProANP has a longer plasma half-life, making it a reliable diagnostic/prognostic indicator in cardiovascular diseases. Recombinant versions are critical for developing immunoassays, calibrating diagnostic kits, and investigating pathophysiological mechanisms.
Current research focuses on optimizing expression systems to ensure proper post-translational modifications and bioactivity. Challenges include maintaining structural stability, avoiding aggregation, and achieving cost-effective large-scale production. Beyond diagnostics, recombinant NT-ProANP is explored for therapeutic potential, such as modulating natriuretic peptide pathways or serving as a decoy receptor target. Its role in early disease detection and personalized medicine continues to drive biotechnological advancements in this field.
以下是3篇关于NT-ProBNP重组蛋白的参考文献概览：
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1. **标题**：Production and characterization of recombinant NT-proBNP for immunoassay development
**作者**：Smith A, et al.
**摘要**：研究团队通过大肠杆菌表达系统成功制备了重组NT-ProBNP蛋白，优化了纯化工艺，并通过质谱和Western blot验证其结构。该蛋白被用于开发高灵敏度的化学发光免疫分析法，为心血管疾病诊断提供工具。
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2. **标题**：Expression of recombinant NT-proBNP in mammalian cells and its clinical utility comparison
**作者**：Zhang L, et al.
**摘要**：在HEK293哺乳动物细胞中表达重组NT-ProBNP，获得糖基化修饰的蛋白，与天然蛋白结构高度一致。研究表明，哺乳动物系统表达的蛋白在临床检测中的稳定性和特异性优于原核表达产物。
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3. **标题**：Development of a NT-proBNP sandwich ELISA using recombinant protein as a calibrator
**作者**：Wang Y, et al.
**摘要**：利用重组NT-ProBNP作为校准品，建立双抗体夹心ELISA检测方法，验证其与商业检测试剂盒的一致性。结果显示重组蛋白作为标准品可提高检测的重复性和准确性，降低临床诊断成本。
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4. **标题**：Recombinant NT-proBNP as a biomarker for heart failure: Analytical validation study
**作者**：Johnson R, et al.
**摘要**：系统评估重组NT-ProBNP在心力衰竭诊断中的性能，通过多中心研究验证其与血浆样本的相关性。研究表明重组蛋白可作为可靠的检测标准物，支持临床实验室标准化进程。
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*注：以上文献信息为示例性质，实际文献需通过学术数据库检索确认。建议使用PubMed或Web of Science以关键词“recombinant NT-proBNP”获取具体文献。*
NT-ProBNP (N-terminal pro-B-type natriuretic peptide) is a biologically inactive peptide fragment derived from the cleavage of prohormone proBNP, which is primarily secreted by cardiac ventricular myocytes in response to myocardial stretch and increased ventricular wall tension. As a key biomarker in cardiovascular diagnostics, NT-ProBNP plays a critical role in assessing heart failure, ventricular dysfunction, and other cardiac conditions. Unlike its counterpart BNP (B-type natriuretic peptide), NT-ProBNP has a longer plasma half-life (60-120 minutes) and greater stability, making it more reliable for clinical immunoassays.
Recombinant NT-ProBNP proteins are engineered using genetic recombination techniques to produce standardized, high-purity antigens for diagnostic and research applications. These proteins are typically expressed in heterologous systems such as *E. coli* or mammalian cell lines, followed by purification via affinity chromatography. The recombinant form retains the epitopes required for antibody recognition in diagnostic kits, ensuring consistency in assays like ELISA or chemiluminescent immunoassays (CLIA).
Clinically, recombinant NT-ProBNP serves as a calibration standard in commercial diagnostic platforms to quantify endogenous NT-ProBNP levels in patient blood. Elevated concentrations correlate with disease severity, aiding in heart failure diagnosis, prognosis, and therapeutic monitoring. In research, it facilitates studies on cardiac biomarker biology, assay development, and the exploration of pathophysiological mechanisms underlying cardiovascular diseases. Its recombinant production addresses batch variability issues inherent in native protein extraction, enhancing reproducibility in both diagnostic and experimental settings.
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