| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SERTAD3 |
| Uniprot No | Q9UJW9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-196 aa |
| 活性数据 | MVGGLKRKHS DLEEEEERWE WSPAGLQSYQ QALLRISLDK VQRSLGPRAP SLRRHVLIHN TLQQLQAALR LAPAPALPPE PLFLGEEDFS LSATIGSILR ELDTSMDGTE PPQNPVTPLG LQNEVPPQPD PVFLEALSSR YLGDSGLDDF FLDIDTSAVE KEPARAPPEP PHNLFCAPGS WEWNELDHIM EIILGS |
| 分子量 | 21.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SERTAD3蛋白的参考文献示例(注意：以下内容为模拟生成，仅供参考)：
1. **文献名称**：**SERTAD3 promotes breast cancer cell proliferation via E2F signaling**
**作者**：Xu, Y. et al. (2015)
**摘要**：研究揭示了SERTAD3在乳腺癌中过表达的机制，通过结合并激活E2F转录因子，调控细胞周期相关基因的表达，促进肿瘤增殖。
2. **文献名称**：**Recombinant expression and structural characterization of human SERTAD3 protein in E. coli**
**作者**：Zhang, L. et al. (2018)
**摘要**：报道了利用大肠杆菌系统高效表达重组人SERTAD3蛋白的方法，并对其结构与功能域进行了生物物理分析。
3. **文献名称**：**SERTAD3 interacts with p53 to enhance its transcriptional activity in apoptosis regulation**
**作者**：Liang, H. et al. (2020)
**摘要**：研究表明SERTAD3与p53蛋白直接结合，增强p53的转录活性，从而促进癌细胞凋亡，揭示了其在癌症治疗中的潜在价值。
4. **文献名称**：**SERTAD3-mediated HPV integration in cervical carcinogenesis**
**作者**：Chen, R. et al. (2017)
**摘要**：探讨了SERTAD3在宫颈癌细胞中与人乳头瘤病毒(HPV)的相互作用，发现其通过调控病毒基因组整合加速肿瘤发生。
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**注**：以上文献信息为模拟生成，实际研究中请通过数据库(如PubMed、Web of Science)检索真实文献。建议搜索关键词：“SERTAD3 function”“SERTAD3 recombinant expression”“SERTAD3 cancer mechanism”等。
SERTAD3 (SERTA domain-containing protein 3) is a member of the SERTA protein family, characterized by the presence of a conserved SERTA domain implicated in transcriptional regulation and protein-protein interactions. Primarily localized in the nucleus, SERTAD3 functions as a transcriptional co-regulator, modulating the activity of key transcription factors such as E2F, which governs cell cycle progression, proliferation, and apoptosis. Structurally, it contains a central SERTA domain and intrinsically disordered regions, enabling dynamic interactions with multiple partners.
Studies suggest SERTAD3 plays roles in cellular stress responses and DNA damage repair. Dysregulation of SERTAD3 is linked to carcinogenesis, with overexpression observed in multiple malignancies, including breast, colorectal, and lung cancers. Elevated SERTAD3 levels correlate with enhanced tumor cell survival, proliferation, and chemoresistance, often indicating poor prognosis. Mechanistically, it may stabilize oncoproteins or inhibit tumor suppressors, though precise pathways remain under investigation.
Recombinant human SERTAD3 protein is typically produced using bacterial or mammalian expression systems, enabling in vitro studies of its interactions, post-translational modifications, and regulatory networks. Its recombinant form facilitates biochemical assays, structural analyses, and drug screening efforts targeting SERTAD3-associated pathways. Ongoing research aims to clarify its physiological roles and therapeutic potential in cancer and stress-related diseases.
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