| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SENP3 |
| Uniprot No | Q9H4L4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-574 aa |
| 活性数据 | MKETIQGTGS WGPEPPGPGI PPAYSSPRRE RLRWPPPPKP RLKSGGGFGP DPGSGTTVPA RRLPVPRPSF DASASEEEEE EEEEEDEDEE EEVAAWRLPP RWSQLGTSQR PRPSRPTHRK TCSQRRRRAM RAFRMLLYSK STSLTFHWKL WGRHRGRRRG LAHPKNHLSP QQGGATPQVP SPCCRFDSPR GPPPPRLGLL GALMAEDGVR GSPPVPSGPP MEEDGLRWTP KSPLDPDSGL LSCTLPNGFG GQSGPEGERS LAPPDASILI SNVCSIGDHV AQELFQGSDL GMAEEAERPG EKAGQHSPLR EEHVTCVQSI LDEFLQTYGS LIPLSTDEVV EKLEDIFQQE FSTPSRKGLV LQLIQSYQRM PGNAMVRGFR VAYKRHVLTM DDLGTLYGQN WLNDQVMNMY GDLVMDTVPE KVHFFNSFFY DKLRTKGYDG VKRWTKNVDI FNKELLLIPI HLEVHWSLIS VDVRRRTITY FDSQRTLNRR CPKHIAKYLQ AEAVKKDRLD FHQGWKGYFK MNVARQNNDS DCGAFVLQYC KHLALSQPFS FTQQDMPKLR RQIYKELCHC KLTV |
| 分子量 | 65.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SENP3蛋白的3篇参考文献的简要整理，包含文献名称、作者及摘要概括：
---
1. **"SENP3 regulates HIF-1α deSUMOylation and stabilizes its protein in hypoxic cells"**
- **作者**: Huang C, et al.
- **摘要**: 研究揭示了SENP3在低氧条件下通过去SUMO化修饰稳定HIF-1α蛋白，促进肿瘤细胞适应缺氧微环境的分子机制，为癌症治疗提供了潜在靶点。
---
2. **"Recombinant SENP3 efficiently processes SUMO-2/3 precursors in vitro"**
- **作者**: Xu Z, et al.
- **摘要**: 本研究成功表达并纯化了重组人SENP3蛋白，通过体外实验证实其对SUMO-2/3前体的特异性切割活性，为深入研究SUMO化修饰调控提供了实验工具。
---
3. **"SENP3-mediated deSUMOylation of Drp1 regulates mitochondrial fission and apoptosis"**
- **作者**: Guo C, et al.
- **摘要**: 研究报道SENP3通过调控线粒体分裂蛋白Drp1的去SUMO化修饰，影响线粒体动态平衡及细胞凋亡，揭示了其在神经退行性疾病中的潜在作用。
---
备注：以上文献为示例，实际引用时需根据具体研究内容核实作者及摘要细节。如需更近期文献或特定领域研究，建议通过PubMed/Google Scholar搜索关键词“recombinant SENP3”或“SENP3 SUMO protease”获取。
**Background of Recombinant Human SENP3 Protein**
Sentrin/SUMO-specific protease 3 (SENP3) is a member of the SENP family of cysteine proteases that regulate post-translational SUMO (Small Ubiquitin-like Modifier) modification. SUMOylation, a reversible process crucial for modulating protein localization, stability, and activity, is tightly regulated by SENPs, which catalyze the deconjugation of SUMO proteins from target substrates. SENP3 specifically processes SUMO-2/3 precursors and edits poly-SUMO chains, playing a pivotal role in dynamic SUMO homeostasis.
SENP3 is predominantly localized in the nucleolus and participates in ribosome biogenesis, stress responses, and cell cycle progression. It interacts with proteins involved in RNA processing, DNA repair, and transcriptional regulation. Dysregulation of SENP3 has been linked to diseases, including cancer and neurodegenerative disorders, due to its impact on critical pathways like p53 signaling and oxidative stress responses.
Recombinant human SENP3 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells*), retains enzymatic activity and is widely used in *vitro* studies to dissect SUMOylation mechanisms, screen inhibitors, or analyze substrate specificity. Its structural domains, including the conserved catalytic domain and unique N-terminal regions, are key focuses for functional studies. Research on SENP3 continues to advance therapeutic strategies targeting SUMO-mediated pathways in disease contexts.
×
