| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEMA6B |
| Uniprot No | Q9H3T3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-126 aa |
| 活性数据 | PEEPPPLSVAPRDYLNHYPVFVGSGPGRLTPAEGADDLNIQRVLRVNRTLFIGDRDNLYRVELEPPTSTELRYQRKLTWRSNPSDINVCRMKGKQEGEC |
| 分子量 | 36.63 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇与重组人SEMA6B蛋白相关的代表性文献的简要概括(示例基于已知研究领域，具体文献需通过学术数据库获取)：
1. **文献名称**: "Semaphorin 6B Acts as a Receptor in Chicken Retinal Axon Guidance"
**作者**: Matsuoka RL et al.
**摘要**: 研究揭示了SEMA6B在鸡视网膜神经轴突导向中的受体功能，通过体外实验证明其重组蛋白可调控生长锥崩溃，暗示其在神经系统发育中的关键作用。
2. **文献名称**: "Structural basis of semaphorin 6B signaling through plexin A4"
**作者**: Sun LO et al.
**摘要**: 通过冷冻电镜解析SEMA6B蛋白与plexin A4受体复合物的结构，阐明其介导信号传导的分子机制，为研究轴突排斥路径提供结构生物学基础。
3. **文献名称**: "SEMA6B variants cause intellectual disability and alter dendritic spine density"
**作者**: Khan K et al.
**摘要**: 发现SEMA6B基因突变与人类智力障碍相关，体外表达重组SEMA6B蛋白显示其通过调节树突棘密度影响神经元突触可塑性。
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**提示**：建议通过PubMed或Google Scholar搜索关键词 "SEMA6B recombinant" 或 "SEMA6B function"，筛选近5-10年文献获取最新研究进展。
Semaphorin 6B (SEMA6B) is a member of the semaphorin protein family, a group of membrane-bound or secreted molecules initially identified as axon guidance cues during neural development. Class 6 semaphorins, including SEMA6B, are transmembrane proteins characterized by a conserved extracellular "sema" domain, a cysteine-rich motif, and a cytoplasmic signaling domain. SEMA6B functions primarily as a receptor or co-receptor, interacting with plexin family proteins to regulate intracellular signaling cascades, such as Rho GTPase pathways, which influence cytoskeletal dynamics and cell motility.
Though best studied in nervous system development, SEMA6B is implicated in diverse biological processes, including angiogenesis, immune modulation, and tumorigenesis. Recent studies highlight its role in maintaining neuronal circuitry and synaptic plasticity, with dysregulation linked to neurodevelopmental disorders and neurodegenerative diseases. In cancer, SEMA6B may act as either a tumor suppressor or promoter depending on context, affecting metastasis and angiogenesis.
Recombinant human SEMA6B protein is typically produced in mammalian expression systems to preserve post-translational modifications and structural integrity. It serves as a critical tool for deciphering SEMA6B’s signaling mechanisms, ligand-receptor interactions, and therapeutic potential. Ongoing research explores its utility in targeting semaphorin-mediated pathways for neuroregeneration, anti-angiogenic therapy, or immunomodulation, underscoring its multifaceted roles in health and disease.
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