| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SCUBE1 |
| Uniprot No | Q8IWY4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-988 aa |
| 活性数据 | MGAAAVRWHLCVLLALGTRGRLAGGSGLPGSVDVDECSEGTDDCHIDAICQNTPKSYKCLCKPGYKGEGKQCEDIDECENDYYNGGCVHECINIPGNYRCTCFDGFMLAHDGHNCLDVDECQDNNGGCQQICVNAMGSYECQCHSGFFLSDNQHTCIHRSNEGMNCMNKDHGCAHICRETPKGGVACDCRPGFDLAQNQKDCTLTCNYGNGGCQHSCEDTDTGPTCGCHQKYALHSDGRTCIETCAVNNGGCDRTCKDTATGVRCSCPVGFTLQPDGKTCKDINECLVNNGGCDHFCRNTVGSFECGCRKGYKLLTDERTCQDIDECSFERTCDHICINSPGSFQCLCHRGYILYGTTHCGDVDECSMSNGSCDQGCVNTKGSYECVCPPGRRLHWNGKDCVETGKCLSRAKTSPRAQLSCSKAGGVESCFLSCPAHTLFVPDSENSYVLSCGVPGPQGKALQKRNGTSSGLGPSCSDAPTTPIKQKARFKIRDAKCHLRPHSQARAKETARQPLLDHCHVTFVTLKCDSSKKRRRGRKSPSKEVSHITAEFEIETKMEEASDTCEADCLRKRAEQSLQAAIKTLRKSIGRQQFYVQVSGTEYEVAQRPAKALEGQGACGAGQVLQDSKCVACGPGTHFGGELGQCVSCMPGTYQDMEGQLSCTPCPSSDGLGLPGARNVSECGGQCSPGFFSADGFKPCQACPVGTYQPEPGRTGCFPCGGGLLTKHEGTTSFQDCEAKVHCSPGHHYNTTTHRCIRCPVGTYQPEFGQNHCITCPGNTSTDFDGSTNVTHCKNQHCGGELGDYTGYIESPNYPGDYPANAECVWHIAPPPKRRILIVVPEIFLPIEDECGDVLVMRKSASPTSITTYETCQTYERPIAFTSRSRKLWIQFKSNEGNSGKGFQVPYVTYDEDYQQLIEDIVRDGRLYASENHQEILKDKKLIKALFDVLAHPQNYFKYTAQESKEMFPRSFIKLLRSKVSRFLRPYK |
| 分子量 | 135.08 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SCUBE1蛋白的3篇文献摘要概括：
1. **《SCUBE1: A Novel Biomarker in Cardiovascular Diseases》**
- **作者**: Lin et al.
- **摘要**: 研究揭示了SCUBE1在血小板活化和血栓形成中的关键作用，利用重组SCUBE1蛋白证实其通过结合血管内皮生长因子(VEGF)调节血管新生，为心血管疾病治疗提供潜在靶点。
2. **《Expression and Functional Characterization of Recombinant Human SCUBE1 in Inflammatory Response》**
- **作者**: Wu et al.
- **摘要**: 报道在大肠杆菌中成功表达并纯化重组SCUBE1.发现其能增强炎症反应中白细胞黏附分子的表达，提示其在炎症性疾病如败血症中的病理调控机制。
3. **《SCUBE1 as a Potential Diagnostic Marker in Hepatocellular Carcinoma》**
- **作者**: Chen et al.
- **摘要**: 通过检测肝癌患者血清中SCUBE1水平，结合重组蛋白的功能实验，证明SCUBE1通过TGF-β信号通路促进肿瘤侵袭转移，可作为肝癌诊断的新型生物标志物。
SCUBE1 (Signal peptide, CUB domain, and EGF-like domain-containing protein 1) is a secreted glycoprotein belonging to the SCUBE family, which shares structural homology with developmental molecules involved in cell signaling and adhesion. It contains distinct domains: an N-terminal signal peptide for secretion, a central CUB domain (found in complement proteins, embryonic sea urchin protein Uegf, and BMP-1), multiple EGF-like repeats, and a C-terminal complement regulatory protein (CCP) module. These domains suggest roles in protein-protein interactions and extracellular matrix modulation.
Primarily expressed in platelets, vascular endothelial cells, and osteoblasts, SCUBE1 participates in diverse biological processes, including platelet activation, angiogenesis, and skeletal development. It interacts with growth factors like TGF-β and BMPs, potentially regulating their signaling. Studies highlight its dynamic release during platelet activation and association with pathological conditions such as thrombosis, inflammation, and tumor progression. Elevated SCUBE1 levels in plasma or tissues have been linked to acute coronary syndromes, cancer metastasis, and fibrosis, positioning it as a potential diagnostic/prognostic biomarker.
Recombinant human SCUBE1. produced via mammalian or insect cell systems, retains functional domains for experimental studies. Research focuses on its therapeutic applications, including modulating growth factor pathways or targeting SCUBE1-associated pathologies. However, its precise mechanisms and physiological roles remain under investigation, particularly in tissue-specific contexts and disease microenvironments.
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