| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTCD2 |
| Uniprot No | Q8WV60 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-388 aa |
| 活性数据 | MVRDSMAAAF RPSNRVLLQA LQILVYPGVG GSGSVSCRCP LGAKRYLLTD NVVKLKEFQQ KKVAVACNLS GTKETYFRNL KKKLTQNKLI LKGELITLLH LCESRDHVEL AKNVIYRYHA ENKNFTLGEY KFGPLFVRLC YELDLEESAV ELMKDQHLRG FFSDSTSFNI LMDMLFIKGK YKSALQVLIE MKNQDVKFTK DTYVLAFAIC YKLNSPESFK ICTTLREEAL LKGEILSRRA SCFAVALALN QNEMAKAVSI FSQIMNPESI ACINLNIIIH IQSNMLENLI KTLKNAAEGN LSKFVKRHVF SEEVLAKVRE KVKDVPALVA KFDEIYGTLH ITGQVTTDSL DAVLCHTPRD RKSHTLLLNK RMVSRRTFQP LSQSLLAE |
| 分子量 | 43.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PTCD2蛋白的3篇参考文献示例，主题涵盖其功能及重组技术(注：以下为虚构示例，实际文献需通过数据库检索验证)：
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1. **标题**: *PTCD2 regulates mitochondrial RNA processing through recombinant expression analysis*
**作者**: Smith J, et al.
**摘要**: 研究通过大肠杆菌系统重组表达人PTCD2蛋白，发现其通过C端结构域结合线粒体RNA，调控16S rRNA的稳定性，缺失导致呼吸链复合体缺陷。
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2. **标题**: *Crystal structure of recombinant human PTCD2 reveals RNA-binding motifs*
**作者**: Chen L, et al.
**摘要**: 解析了重组PTCD2的晶体结构(2.8Å)，揭示了其N端PPR重复模体特异性识别RNA茎环结构，为RNA代谢疾病的突变研究提供结构依据。
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3. **标题**: *Functional interaction of PTCD2 with mitochondrial transcription machinery*
**作者**: Wang Y, et al.
**摘要**: 利用HEK293细胞表达重组PTCD2.结合免疫共沉淀实验，证实其与线粒体转录因子TFAM互作，协调mtDNA转录与RNA加工，维持氧化磷酸化功能。
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**提示**：建议使用PubMed或Google Scholar，以“PTCD2 recombinant protein”或“PTCD2 mitochondrial RNA”为关键词检索近期文献，关注《Nucleic Acids Research》《Biochimica et Biophysica Acta》等期刊。
Recombinant human PTCD2 (Pentatricopeptide Repeat Domain 2) protein is a mitochondrial protein involved in RNA metabolism and mitochondrial gene regulation. Structurally, PTCD2 contains pentatricopeptide repeat (PPR) motifs, which enable sequence-specific interactions with RNA molecules. It localizes to the mitochondrial matrix and plays a role in processing mitochondrial RNA (mtRNA), including the stabilization and maturation of mitochondrial transcripts. Studies suggest its importance in maintaining mitochondrial function, particularly in energy-demanding tissues like the heart and brain.
PTCD2 is implicated in mitochondrial disorders and cellular stress responses. Reduced PTCD2 expression has been linked to impaired assembly of mitochondrial respiratory chain complexes, leading to oxidative phosphorylation defects and increased reactive oxygen species (ROS). This dysfunction may contribute to pathologies such as cardiomyopathy, neurodegenerative diseases, and metabolic syndromes. For instance, PTCD2 deficiency in cardiac cells disrupts mtRNA processing, exacerbating mitochondrial dysfunction during heart failure. Additionally, PTCD2 interacts with microRNAs and stress-response pathways, highlighting its regulatory role in cell survival and apoptosis.
Recombinant PTCD2 protein is widely used to study mitochondrial RNA-protein interactions, disease mechanisms, and therapeutic strategies targeting mitochondrial dysfunction. Its applications range from in vitro RNA-binding assays to cellular models of mitochondrial diseases, aiding in the exploration of gene therapy or small-molecule interventions. Ongoing research focuses on elucidating its post-translational modifications and tissue-specific roles in health and disease.
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