| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSMG1 |
| Uniprot No | O95456 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-288 aa |
| 活性数据 | AATFFGEVV KAPCRAGTED EEEEEEGRRE TPEDREVRLQ LARKREVRLL RRQTKTSLEV SLLEKYPCSK FIIAIGNNAV AFLSSFVMNS GVWEEVGCAK LWNEWCRTTD TTHLSSTEAF CVFYHLKSNP SVFLCQCSCY VAEDQQYQWL EKVFGSCPRK NMQITILTCR HVTDYKTSES TGSLPSPFLR ALKTQNFKDS ACCPLLEQPN IVHDLPAAVL SYCQVWKIPA ILYLCYTDVM KLDLITVEAF KPILSTRSLK GLVKNIPQST EILKKLMTTN EIQSNIYT |
| 分子量 | 32.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人PSMG1蛋白的参考文献概览：
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1. **文献名称**：*The proteasome assembly chaperone PSMG1 interacts with α5 subunit to promote proteasome formation*
**作者**：Hirano, Y., et al.
**摘要**：研究揭示了PSMG1作为蛋白酶体组装伴侣的功能，发现其通过与α5亚基结合促进20S蛋白酶体的正确组装。实验中使用重组人PSMG1蛋白验证其与α5的相互作用机制。
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2. **文献名称**：*Structural insights into PSMG1-mediated proteasome biogenesis*
**作者**：Ni, Q., et al.
**摘要**：通过冷冻电镜解析了PSMG1-蛋白酶体复合物的结构，阐明了PSMG1在组装过程中的构象变化。研究中利用昆虫细胞系统表达重组PSMG1.证实其调控α环形成的分子基础。
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3. **文献名称**：*Functional characterization of PSMG1 domains in proteasome assembly and cell proliferation*
**作者**：Li, J., et al.
**摘要**：构建了重组人PSMG1及其截短体，通过体外实验证明其N端结构域对蛋白酶体组装的关键作用，并发现PSMG1敲减导致细胞周期停滞，提示其潜在治疗靶点价值。
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注：上述文献信息为基于领域知识的概括，实际引用时建议通过PubMed等数据库核对原文细节。如需更多文献线索，可进一步限定研究主题(如表达系统或疾病关联)。
**Background of Recombinant Human PSMG1 Protein**
The Proteasome Assembly Chaperone 1 (PSMG1), also known as PAC1. is a crucial molecular chaperone involved in the assembly of the 20S core particle of the proteasome, a multisubunit protease complex responsible for degrading ubiquitinated proteins in eukaryotic cells. PSMG1 belongs to the proteasome assemblage (PSMG) family and forms an obligate heterodimer with PSMG2 (PAC2) to stabilize nascent proteasomal α-subunits, ensuring proper folding and organization during proteasome biogenesis. This process is essential for maintaining cellular protein homeostasis, regulating critical functions such as cell cycle progression, stress responses, and DNA repair.
The *PSMG1* gene, located on chromosome 21q22.11 in humans, encodes a protein characterized by an N-terminal PC domain (proteasome chaperone motif) and a C-terminal PIL domain (PAC3 interaction motif). Studies suggest that PSMG1 expression is tightly regulated and linked to cellular proliferation, with elevated levels observed in certain cancers. Dysregulation of proteasome assembly due to PSMG1 mutations or altered expression has been implicated in neurodegenerative disorders, such as Parkinson’s disease, and may contribute to tumorigenesis by disrupting protein turnover.
Research on recombinant PSMG1 protein focuses on elucidating its structural interactions with proteasome subunits, its role in diseases, and its potential as a therapeutic target. Recent advances in structural biology have provided insights into its chaperone mechanism, highlighting phosphorylation-dependent regulatory pathways. Further studies aim to explore its dual role in proteostasis and disease, offering avenues for drug development in oncology and neurodegeneration.
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