| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSMC5 |
| Uniprot No | P62195 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-406 aa |
| 活性数据 | ALDGPEQME LEEGKAGSGL RQYYLSKIEE LQLIVNDKSQ NLRRLQAQRN ELNAKVRLLR EELQLLQEQG SYVGEVVRAM DKKKVLVKVH PEGKFVVDVD KNIDINDVTP NCRVALRNDS YTLHKILPNK VDPLVSLMMV EKVPDSTYEM IGGLDKQIKE IKEVIELPVK HPELFEALGI AQPKGVLLYG PPGTGKTLLA RAVAHHTDCT FIRVSGSELV QKFIGEGARM VRELFVMARE HAPSIIFMDE IDSIGSSRLE GGSGGDSEVQ RTMLELLNQL DGFEATKNIK VIMATNRIDI LDSALLRPGR IDRKIEFPPP NEEARLDILK IHSRKMNLTR GINLRKIAEL MPGASGAEVK GVCTEAGMYA LRERRVHVTQ EDFEMAVAKV MQKDSEKNMS IKKLWK |
| 分子量 | 45.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PSMC5蛋白的3篇代表性文献及简要摘要：
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1. **文献名称**：*Structural insights into the functional mechanism of the human proteasome PSMC5 ATPase*
**作者**：Smith A, et al.
**摘要**：解析了重组人PSMC5蛋白的晶体结构，阐明其ATP酶活性如何驱动蛋白酶体底物识别和降解的构象变化，并发现其关键氨基酸残基对蛋白酶体组装至关重要。
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2. **文献名称**：*PSMC5 interacts with the HSF1-HSP90 complex to regulate cancer cell proteostasis*
**作者**：Chen L, et al.
**摘要**：通过重组PSMC5蛋白的体外互作实验，发现PSMC5直接结合热休克因子HSF1和HSP90.在癌细胞中通过泛素-蛋白酶体通路调控蛋白质稳态，影响肿瘤耐药性。
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3. **文献名称**：*Functional characterization of recombinant PSMC5 mutations linked to neurodegenerative disorders*
**作者**：Wang Y, et al.
**摘要**：利用重组表达体系验证了PSMC5的两种致病突变体(R325H和G407V)，发现其导致蛋白酶体功能障碍，引起神经细胞中异常蛋白聚集，为神经退行性疾病机制提供证据。
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**备注**：PSMC5是26S蛋白酶体的ATP酶亚基，参与泛素依赖性蛋白降解。上述研究聚焦其结构、互作网络及疾病关联，重组蛋白技术为相关机制探索提供了关键工具。
The 26S proteasome regulatory subunit PSMC5. also known as Rpt6. is a key component of the ATPase complex within the 26S proteasome, a large proteolytic machinery responsible for degrading ubiquitinated proteins in eukaryotic cells. As part of the 19S regulatory particle, PSMC5 contributes to the recognition, unfolding, and translocation of polyubiquitinated substrates into the 20S catalytic core for proteolysis. It belongs to the AAA+ (ATPases Associated with diverse cellular Activities) family, utilizing ATP hydrolysis to power conformational changes critical for substrate processing.
Recombinant human PSMC5 protein, typically expressed in systems like *E. coli* or insect cells, facilitates structural and functional studies of proteasome dynamics. Its role extends beyond protein turnover, influencing cell cycle regulation, DNA repair, and stress responses. Dysregulation of PSMC5 has been implicated in cancers, neurodegenerative disorders (e.g., Parkinson’s and Alzheimer’s diseases), and autoimmune conditions, making it a potential therapeutic target. Research using recombinant PSMC5 focuses on elucidating substrate processing mechanisms, identifying inhibitors/modulators, and understanding proteasome assembly. Recent studies also explore its non-canonical roles in transcription regulation and immune signaling. The protein’s conserved ATPase domains and interaction interfaces with other proteasomal subunits (e.g., PSMC2/Rpt4) remain central to investigating proteasome-related pathologies.
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