| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRPF4 |
| Uniprot No | O43172 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-522 aa |
| 活性数据 | MASSRASSTQ ATKTKAPDDL VAPVVKKPHI YYGSLEEKER ERLAKGESGI LGKDGLKAGI EAGNINITSG EVFEIEEHIS ERQAEVLAEF ERRKRARQIN VSTDDSEVKA CLRALGEPIT LFGEGPAERR ERLRNILSVV GTDALKKTKK DDEKSKKSKE EYQQTWYHEG PNSLKVARLW IANYSLPRAM KRLEEARLHK EIPETTRTSQ MQELHKSLRS LNNFCSQIGD DRPISYCHFS PNSKMLATAC WSGLCKLWSV PDCNLLHTLR GHNTNVGAIV FHPKSTVSLD PKDVNLASCA ADGSVKLWSL DSDEPVADIE GHTVRVARVM WHPSGRFLGT TCYDRSWRLW DLEAQEEILH QEGHSMGVYD IAFHQDGSLA GTGGLDAFGR VWDLRTGRCI MFLEGHLKEI YGINFSPNGY HIATGSGDNT CKVWDLRQRR CVYTIPAHQN LVTGVKFEPI HGNFLLTGAY DNTAKIWTHP GWSPLKTLAG HEGKVMGLDI SSDGQLIATC SYDRTFKLWM AE |
| 分子量 | 58.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PRPF4蛋白的3条参考文献，基于合理推测整理(若需真实文献，建议通过学术数据库检索)：
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1. **文献名称**: *Structural insights into the role of PRPF4 in the U4/U6 snRNP assembly*
**作者**: Chen L, et al.
**摘要**: 通过X射线晶体学解析了重组人PRPF4蛋白与U4/U6 snRNA复合物的结构，揭示了PRPF4通过C端螺旋稳定snRNP构象的机制，为剪接体早期组装提供结构基础。
2. **文献名称**: *PRPF4 mutations disrupt retinal splicing and cause autosomal dominant retinitis pigmentosa*
**作者**: Zhao H, et al.
**摘要**: 研究发现在视网膜色素变性患者中，PRPF4基因的错义突变导致重组蛋白异常聚集，并通过破坏pre-mRNA剪接关键因子的招募，引发光感受器细胞凋亡。
3. **文献名称**: *Recombinant PRPF4 expression and purification for high-throughput drug screening*
**作者**: Kim S, et al.
**摘要**: 开发了一种基于大肠杆菌的重组人PRPF4蛋白高效表达及纯化系统，结合体外剪接活性实验，用于靶向剪接体功能异常相关疾病的化合物筛选。
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注：上述文献为示例性内容，实际研究中请通过PubMed、Web of Science等平台获取真实文献。
**Background of Recombinant Human PRPF4 Protein**
PRPF4 (Pre-mRNA Processing Factor 4) is a key component of the spliceosome, a dynamic macromolecular complex responsible for precise removal of introns during mRNA splicing. It plays a critical role in assembling and stabilizing the U4/U6.U5 tri-snRNP particle, essential for spliceosome activation and catalytic steps. Structurally, PRPF4 contains tetratricopeptide repeat (TPR) domains, facilitating protein-protein interactions within the spliceosome network.
Dysregulation of PRPF4 is implicated in spliceosomopathies, including retinal degeneration and cancer. Mutations in PRPF4 are linked to autosomal dominant retinitis pigmentosa (adRP), highlighting its importance in maintaining retinal homeostasis. In oncology, aberrant splicing driven by PRPF4 dysexpression may contribute to tumor progression.
Recombinant human PRPF4 protein, produced via expression systems like *E. coli* or mammalian cells, enables functional studies of splicing mechanisms and disease-related variants. Its applications span *in vitro* splicing assays, structural analyses, and drug screening platforms targeting spliceosomal disorders. Research on recombinant PRPF4 advances understanding of splicing regulation and therapeutic strategies for spliceosome-associated diseases.
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