| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRKCE |
| Uniprot No | P17252 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-672 aa |
| 活性数据 | ADVFPGNDS TASQDVANRF ARKGALRQKN VHEVKDHKFI ARFFKQPTFC SHCTDFIWGF GKQGFQCQVC CFVVHKRCHE FVTFSCPGAD KGPDTDDPRS KHKFKIHTYG SPTFCDHCGS LLYGLIHQGM KCDTCDMNVH KQCVINVPSL CGMDHTEKRG RIYLKAEVAD EKLHVTVRDA KNLIPMDPNG LSDPYVKLKL IPDPKNESKQ KTKTIRSTLN PQWNESFTFK LKPSDKDRRL SVEIWDWDRT TRNDFMGSLS FGVSELMKMP ASGWYKLLNQ EEGEYYNVPI PEGDEEGNME LRQKFEKAKL GPAGNKVISP SEDRKQPSNN LDRVKLTDFN FLMVLGKGSF GKVMLADRKG TEELYAIKIL KKDVVIQDDD VECTMVEKRV LALLDKPPFL TQLHSCFQTV DRLYFVMEYV NGGDLMYHIQ QVGKFKEPQA VFYAAEISIG LFFLHKRGII YRDLKLDNVM LDSEGHIKIA DFGMCKEHMM DGVTTRTFCG TPDYIAPEII AYQPYGKSVD WWAYGVLLYE MLAGQPPFDG EDEDELFQSI MEHNVSYPKS LSKEAVSVCK GLMTKHPAKR LGCGPEGERD VREHAFFRRI DWEKLENREI QPPFKPKVCG KGAENFDKFF TRGQPVLTPP DQLVIANIDQ SDFEGFSYVN PQFVHPILQS AV |
| 分子量 | 76.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PRKCE蛋白的3-4条参考文献示例(部分内容为模拟文献，供参考)：
1. **文献名称**: *Expression and Purification of Recombinant Human PKCε in a Baculovirus System*
**作者**: Smith J, et al. (2015)
**摘要**: 该研究报道了使用杆状病毒表达系统高效表达重组人PRKCE蛋白，并通过色谱技术实现纯化，证实其具有ATP结合和激酶活性功能。
2. **文献名称**: *PKCε-Mediated Cardioprotection in Ischemic Preconditioning: Role of Recombinant Protein Delivery*
**作者**: Jones R, et al. (2018)
**摘要**: 通过体外递送重组人PRKCE蛋白至心肌细胞，发现其能够激活线粒体KATP通道，显著减少缺血再灌注损伤，揭示了PRKCE在心脏保护中的分子机制。
3. **文献名称**: *Structural Insights into PKCε Kinase Domain via X-ray Crystallography*
**作者**: Zhang L, et al. (2022)
**摘要**: 本研究解析了重组人PRKCE激酶结构域的晶体结构，揭示了其与抑制剂相互作用的活性位点，为靶向PRKCE的药物设计提供了结构基础。
4. **文献名称**: *PKCε Promotes Cancer Cell Invasion via Recombinant Protein-Induced Signaling Pathways*
**作者**: Lee S, et al. (2020)
**摘要**: 利用重组PRKCE蛋白处理乳腺癌细胞，发现其通过激活RhoA/ROCK通路增强细胞迁移和侵袭能力，提示PRKCE可能是癌症治疗的潜在靶点。
(注：以上文献为模拟示例，实际引用请核实真实数据库。)
Protein kinase C epsilon (PRKCE), a member of the protein kinase C (PKC) family, is a serine/threonine kinase that plays a critical role in cellular signaling pathways. It belongs to the novel PKC subfamily (nPKC), characterized by calcium-independent activation but dependence on diacylglycerol (DAG) and phospholipids. PRKCE is encoded by the *PRKCE* gene in humans and is widely expressed in tissues, including the brain, heart, and immune cells. Structurally, it contains a regulatory domain with tandem C1 domains for DAG binding and a catalytic domain for phosphorylation activity.
PRKCE regulates diverse physiological processes, such as cell proliferation, apoptosis, differentiation, and survival. It is implicated in cardiac hypertrophy, neuroprotection, insulin signaling, and immune responses. Dysregulation of PRKCE has been linked to pathologies, including cancer (e.g., breast, prostate), neurodegenerative diseases, and cardiovascular disorders. Its role in cancer involves modulating tumor cell migration, invasion, and chemoresistance.
Recombinant human PRKCE protein is engineered using expression systems (e.g., *E. coli*, mammalian cells) to study its enzymatic activity, interactions, and signaling mechanisms. It serves as a tool for *in vitro* kinase assays, inhibitor screening, and structure-function studies. Researchers also explore its therapeutic potential as a drug target, particularly in oncology and inflammation-related diseases. Understanding PRKCE's molecular dynamics remains vital for deciphering its context-dependent roles and developing targeted therapies.
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