| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRKCA |
| Uniprot No | P17252 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-672 aa |
| 活性数据 | MADVFPGNDSTASQDVANRFARKGALRQKNVHEVKDHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPDTDDPRSKHKFKIHTYGSPTFCDHCGSLLYGLIHQGMKCDTCDMNVHKQCVINVPSLCGMDHTEKRGRIYLKAEVADEKLHVTVRDAKNLIPMDPNGLSDPYVKLKLIPDPKNESKQKTKTIRSTLNPQWNESFTFKLKPSDKDRRLSVEIWDWDRTTRNDFMGSLSFGVSELMKMPASGWYKLLNQEEGEYYNVPIPEGDEEGNMELRQKFEKAKLGPAGNKVISPSEDRKQPSNNLDRVKLTDFNFLMVLGKGSFGKVMLADRKGTEELYAIKILKKDVVIQDDDVECTMVEKRVLALLDKPPFLTQLHSCFQTVDRLYFVMEYVNGGDLMYHIQQVGKFKEPQAVFYAAEISIGLFFLHKRGIIYRDLKLDNVMLDSEGHIKIADFGMCKEHMMDGVTTRTFCGTPDYIAPEIIAYQPYGKSVDWWAYGVLLYEMLAGQPPFDGEDEDELFQSIMEHNVSYPKSLSKEAVSICKGLMTKHPAKRLGCGPEGERDVREHAFFRRIDWEKLENREIQPPFKPKVCGKGAENFDKFFTRGQPVLTPPDQLVIANIDQSDFEGFSYVNPQFVHPILQSAV |
| 分子量 | 103.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PRKCA蛋白的参考文献示例(仅供参考，内容为示例性概括)：
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1. **文献名称**：*Structural and Functional Characterization of Recombinant Human Protein Kinase C Alpha*
**作者**：Smith J, Doe R, Lee T
**摘要**：该研究通过哺乳动物表达系统成功表达了重组人PRKCA蛋白，并利用晶体学方法解析了其三维结构。结果表明，重组PRKCA具有与天然蛋白相似的激酶活性，且其调控结构域对钙离子和脂质结合敏感。
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2. **文献名称**：*Role of Recombinant PRKCA in Cancer Cell Signaling Pathways*
**作者**：Chen L, Wang H, Garcia M
**摘要**：通过体外重组表达PRKCA蛋白，研究揭示了其在MAPK/ERK信号通路中的关键作用。实验发现，重组PRKCA可激活下游促癌基因表达，并促进肿瘤细胞的迁移和侵袭。
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3. **文献名称**：*Optimization of Recombinant PRKCA Expression in E. coli for Drug Screening*
**作者**：Kim S, Patel A, Zhang Y
**摘要**：本研究优化了PRKCA在大肠杆菌中的可溶性表达条件，实现了高效重组蛋白生产。重组蛋白被用于高通量筛选激酶抑制剂，成功鉴定出两种新型抗肿瘤候选化合物。
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4. **文献名称**：*Recombinant PRKCA as a Therapeutic Target in Neurodegenerative Diseases*
**作者**：Müller F, Rossi D, Alvarez G
**摘要**：通过重组PRKCA蛋白的体外实验和动物模型，研究证明其异常磷酸化与阿尔茨海默病相关。抑制重组PRKCA活性可减少神经元凋亡，提示其作为治疗靶点的潜力。
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**注**：以上文献信息为示例，若需真实文献，建议通过PubMed或Google Scholar检索关键词“recombinant PRKCA protein”获取。
Protein kinase C alpha (PRKCA), a member of the protein kinase C (PKC) family, is a serine/threonine kinase that plays a pivotal role in cellular signal transduction. It regulates diverse processes, including cell proliferation, differentiation, apoptosis, and immune responses. PRKCA is activated by calcium, phospholipids, and diacylglycerol (DAG), linking extracellular signals to intracellular pathways such as the MAPK/ERK and PI3K/AKT cascades. Dysregulation of PRKCA is implicated in cancers, cardiovascular diseases, and neurological disorders, making it a therapeutic target.
Recombinant human PRKCA protein is produced using expression systems like *E. coli* or mammalian cells, ensuring proper post-translational modifications for functional activity. This engineered protein retains the catalytic domain and regulatory regions necessary for kinase activity, enabling *in vitro* studies of enzyme kinetics, substrate interactions, and inhibitor screening. Researchers utilize it to dissect PRKCA's role in disease mechanisms, validate drug candidates, and explore signaling network crosstalk. Its availability in purified form accelerates structural studies (e.g., crystallography) and high-throughput assays, bridging basic research and translational applications.
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