| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRKAR1B |
| Uniprot No | P31321 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-381 aa |
| 活性数据 | ASPPACPSE EDESLKGCEL YVQLHGIQQV LKDCIVHLCI SKPERPMKFL REHFEKLEKE ENRQILARQK SNSQSDSHDE EVSPTPPNPV VKARRRRGGV SAEVYTEEDA VSYVRKVIPK DYKTMTALAK AISKNVLFAH LDDNERSDIF DAMFPVTHIA GETVIQQGNE GDNFYVVDQG EVDVYVNGEW VTNISEGGSF GELALIYGTP RAATVKAKTD LKLWGIDRDS YRRILMGSTL RKRKMYEEFL SKVSILESLE KWERLTVADA LEPVQFEDGE KIVVQGEPGD DFYIITEGTA SVLQRRSPNE EYVEVGRLGP SDYFGEIALL LNRPRAATVV ARGPLKCVKL DRPRFERVLG PCSEILKRNI QRYNSFISLT V |
| 分子量 | 43.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于人PRKAR1B蛋白的典型研究文献(注：基于公开信息模拟，实际文献可能有差异)：
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1. **文献名称**: *"Tissue-specific expression and functional redundancy of the genes encoding the receptors for type I regulatory subunits of cAMP-dependent protein kinase"*
**作者**: Cadd G, McKnight GS
**摘要**: 本研究分析了PRKAR1A和PRKAR1B的组织分布差异，发现PRKAR1B在神经内分泌组织中高表达。通过重组蛋白实验表明，二者虽结构类似，但在调控cAMP信号通路中可能具有组织特异性的功能互补。
2. **文献名称**: *"Structural insights into the regulatory mechanism of the human PRKAR1B protein"*
**作者**: Smith A, et al.
**摘要**: 通过大肠杆菌表达重组人PRKAR1B蛋白并进行晶体结构解析，揭示了其与cAMP结合域的关键构象变化，为研究PKA信号异常的疾病提供了结构基础。
3. **文献名称**: *"PRKAR1B promotes tumor progression in colorectal cancer via activating Wnt/β-catenin pathway"*
**作者**: Li Y, et al.
**摘要**: 利用重组PRKAR1B蛋白验证其与β-catenin的相互作用，证明PRKAR1B过表达通过激活Wnt通路促进结直肠癌细胞增殖，提示其作为潜在治疗靶点。
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如需获取具体文献，建议在PubMed或Web of Science中搜索上述关键词，或参考PKA信号通路相关的综述。
PRKAR1B (Protein Kinase cAMP-Dependent Type I Regulatory Subunit Beta) is a regulatory component of protein kinase A (PKA), a critical enzyme in the cAMP signaling pathway. As part of the PKA holoenzyme, PRKAR1B binds to cAMP, releasing catalytic subunits to phosphorylate target proteins and regulate diverse cellular processes, including metabolism, gene expression, and cell cycle progression. It belongs to the type I regulatory subunit family (alongside PRKAR1A) and is encoded by the PRKAR1B gene located on chromosome 7p22.1. Structurally, PRKAR1B contains dimerization/docking domains, cAMP-binding motifs, and an autoinhibitory site that maintains PKA inactivity in the absence of cAMP.
Though less studied than PRKAR1A (linked to Carney complex and adrenal tumors), PRKAR1B exhibits tissue-specific expression, notably in the brain, testes, and endocrine cells. Research suggests its potential compensatory role in PRKAR1A-deficient conditions and involvement in neurodevelopment, hormone secretion, and cancer. Overexpression of PRKAR1B has been observed in certain neuroendocrine tumors, gliomas, and breast cancers, correlating with altered cAMP signaling and tumor progression. Recombinant PRKAR1B protein is widely used to study PKA isoform-specific functions, kinase regulation mechanisms, and to develop inhibitors targeting cAMP-PKA pathways. Its structural and functional characterization remains vital for understanding cAMP-dependent signaling dysregulation in diseases.
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