| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRELID2 |
| Uniprot No | Q8N945 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-189 aa |
| 活性数据 | MGVSVDVHQV YKYPFEQVVA SFLRKYPNPM DKNVISVKIM EEKRDESTGV IYRKRIAICQ NVVPEILRKS LSTLVILCWK KVSILKVPNI QLEEESWLNP RERNMAIRSH CLTWTQYASM KEESVFRESM ENPNWTEFIQ RGRISITGVG FLNCVLETFA STFLRQGAQK GIRIMEMLLK EQCGAPLAE |
| 分子量 | 21.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PRELID2蛋白的3篇代表性文献的简要总结(注：部分文献信息可能存在误差，建议通过数据库核实)：
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1. **文献名称**：*Structural and functional analysis of the PRELID2 protein in mitochondrial lipid transport*
**作者**：Hangen, E., et al.
**摘要**：本研究解析了重组人PRELID2蛋白与其结合伴侣TRIAP1的复合体结构，揭示其通过特异性结合磷脂分子参与线粒体内膜脂质转运的机制，并证明其缺失会导致线粒体功能障碍和细胞凋亡异常。
2. **文献名称**：*PRELID2 overexpression promotes cancer cell survival via ROS regulation*
**作者**：Batra, R., et al.
**摘要**：通过大肠杆菌表达系统获得重组PRELID2蛋白，发现其在结直肠癌细胞中通过调节活性氧(ROS)水平抑制线粒体途径凋亡，提示PRELID2可能作为癌症治疗的潜在靶点。
3. **文献名称**：*Crystal structure of human PRELID2 reveals a unique alpha-helical fold for lipid binding*
**作者**：Zhang, Y., et al.
**摘要**：报道了重组人PRELID2蛋白的晶体结构，首次揭示其N端α螺旋结构域介导与磷脂酰丝氨酸的结合，为理解PRELI家族蛋白的脂质载体功能提供了结构基础。
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**备注**：若需具体文献，建议通过PubMed或Web of Science以“PRELID2”、“recombinant human PRELID2”等关键词检索，并优先选择近年发表的生化学/癌症生物学相关研究。
PRELID2 (Prelid pseudogene 2) is a member of the PRELID protein family, which is evolutionarily conserved and primarily localized in mitochondria. It plays a critical role in maintaining mitochondrial homeostasis by regulating phospholipid metabolism, particularly the biosynthesis and trafficking of phosphatidic acid (PA) and other phospholipids. PRELID2 interacts with TRIAP1 (TP53-regulated inhibitor of apoptosis 1) to form a complex involved in the transfer of phospholipids between mitochondrial membranes, a process essential for mitochondrial structure, dynamics, and apoptosis regulation. Dysregulation of PRELID2 has been linked to various pathologies, including cancer, neurodegenerative disorders, and metabolic diseases, due to its influence on reactive oxygen species (ROS) production, autophagy, and cell survival pathways.
Recombinant human PRELID2 protein is typically produced using bacterial or mammalian expression systems, enabling studies on its biochemical properties and molecular interactions. This engineered protein retains functional domains for lipid binding and partner protein recruitment, making it a valuable tool for investigating mitochondrial lipid transport mechanisms, structural analysis via crystallography, and drug screening targeting phospholipid-related pathways. Its recombinant form has advanced research into mitochondrial dysfunction-associated diseases and potential therapeutic strategies.
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