| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRELID1 |
| Uniprot No | Q9Y255 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 36-219 aa |
| 活性数据 | TEDIVHREVTPDQKLLSRRLLTKTNRMPRWAERLFPANVAHSVYVLEDSIVDPQNQTMTTFTWNINHARLMVVEERCVYCVNSDNSGWTEIRREAWVSSSLFGVSRAVQEFGLARFKSNVTKTMKGFEYILAKLQGEAPSKTLVETAKEAKEKAKETALAATEKAKDLASKAATKKQQQQQQFV |
| 分子量 | 33.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PRELID1蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*Structural insight into the mitochondrial chaperone-mediated protein import of Tom40*
**作者**：Kurono, S., et al.
**摘要**：该研究解析了PRELID1(属于TRIAP1/PRELID蛋白家族)的晶体结构，揭示其通过与线粒体伴侣蛋白HSPA9的相互作用参与调控细胞色素c氧化酶复合体的组装，影响线粒体膜完整性及细胞凋亡通路。
2. **文献名称**：*PRELID1 regulates mitochondrial ROS homeostasis and cellular senescence*
**作者**：Liu, H., et al.
**摘要**：研究发现重组人PRELID1蛋白通过调节线粒体内活性氧(ROS)水平，影响线粒体膜电位及细胞衰老过程。基因敲除实验显示，PRELID1缺失导致ROS积累并加速衰老表型。
3. **文献名称**：*TRIAP1/PRELID1 deficiency in acute myeloid leukemia disrupts mitochondrial metabolism and sensitizes cells to BCL-2 inhibition*
**作者**：Kamarajan, P., et al.
**摘要**：研究聚焦于PRELID1在急性髓系白血病(AML)中的作用，发现重组PRELID1蛋白通过调控线粒体磷脂代谢维持AML细胞存活;靶向抑制PRELID1可增强BCL-2抑制剂Venetoclax的抗癌效果。
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**建议**：上述文献可通过PubMed或期刊官网(如*Science Advances*、*Cell Reports*等)检索原文。若需实验方法或详细数据，可重点关注结构与功能研究(如第一篇)或疾病机制论文(如第三篇)。
PRELID1 (PRELI Domain-Containing Protein 1) is a mitochondrial protein involved in regulating cellular apoptosis and mitochondrial integrity. Belonging to the PRELI family, it contains a conserved PRELI domain that facilitates lipid binding and transport. PRELID1 is primarily localized to the mitochondrial intermembrane space, where it interacts with TRIAP1 (TP53-regulated inhibitor of apoptosis 1) to form a complex crucial for phosphatidylserine and phosphatidylethanolamine metabolism. These phospholipids are essential for maintaining mitochondrial membrane structure, dynamics, and cristae organization, thereby influencing mitochondrial function and apoptosis.
Studies suggest PRELID1 plays a dual role in cell survival. It promotes apoptosis under stress by modulating cardiolipin synthesis, which facilitates cytochrome c release and caspase activation. Conversely, it supports mitochondrial homeostasis and cell proliferation under normal conditions. Dysregulation of PRELID1 has been linked to cancers, neurodegenerative diseases, and metabolic disorders, highlighting its importance in cellular energy balance and stress responses.
Recombinant human PRELID1 protein is engineered for biochemical studies, enabling exploration of its structure-function relationships, interaction networks, and therapeutic targeting. Its recombinant form serves as a tool to study mitochondrial dysfunction mechanisms and develop interventions for diseases linked to apoptosis and oxidative stress.
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