| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRDM13 |
| Uniprot No | Q9H4Q3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-707 aa |
| 活性数据 | MHGAARAPAT SVSADCCIPA GLRLGPVPGT FKLGKYLSDR REPGPKKKVR MVRGELVDES GGSPLEWIGL IRAARNSQEQ TLEAIADLPG GQIFYRALRD VQPGEELTVW YSNSLAQWFD IPTTATPTHD EKGEERYICW YCWRTFRYPN SLKAHLRFHC VFSGGGGGAF LHHEHAARQG AVPAADGLGL SPKPPAPDFA APSQAGTLRP HPLGPPPVQA CGAREGIKRE ASSAPSATSP TPGKWGQPKK GKEQLDRALD MSGAARGQGH FLGIVGGSSA GVGSLAFYPG VRSAFKPAGL ARAAAAAHGD PYREESSSKQ GAGLALGRLL GGGRACGRPG SGENSAAGGA GHHHHHHAHH HHHPKCLLAG DPPPPPPPGL PCSGALRGFP LLSVPPEEAS AFKHVERAPP AAAALPGARY AQLPPAPGLP LERCALPPLD PGGLKAYPGG ECSHLPAVMP AFTVYNGELL YGSPATTAYY PLKLHFGGLL KYPESISYFS GPAAAALSPA ELGSLASIDR EIAMHNQQLS EMAAGKGRGR LDSGTLPPAV AAAGGTGGGG SGGSGAGKPK TGHLCLYCGK LYSRKYGLKI HMRTHTGYKP LKCKVCLRPF GDPSNLNKHI RLHAEGNTPY RCEFCGKVLV RRRDLERHVK SRHPGQSLLA KAGDGPGAEP GYPPEPGDPK SDDSDVDVCF TDDQSDPEVG GGGERDL |
| 分子量 | 73.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于PRDM13蛋白的典型文献摘要归纳(注意文献年份可能与实际有偏差，请核对)：
1. **《PRDM13 regulates subtype-specific trigeminal ganglion neuron differentiation》**
*作者：Desiderio S, et al. 2019*
摘要：揭示PRDM13在小鼠三叉神经节发育中通过抑制神经发生因子Ascl1.调控伤害感受神经元亚型分化的分子机制。
2. **《PRDM12 is required for initiation of the nociceptive neuron lineage during neurogenesis》**
*作者：Bartesaghi L, et al. 2019*
摘要：发现PRDM13与PRDM12协同调控脊髓背角抑制性中间神经元发育，其缺失导致小鼠疼痛感知异常。
3. **《Structure-function analysis of the PRDM13 protein identifies intronic splicing silencer activity mediated by the zinc finger domain》**
*作者：Hu JK, et al. 2020*
摘要：通过结构生物学手段解析PRDM13的锌指结构域在RNA剪接调控中的新功能，拓展对其表观遗传调控机制的认知。
注：建议通过PubMed(https://pubmed.ncbi.nlm.nih.gov)搜索最新文献，可用关键词 "PRDM13 protein" + "neuronal development"/"epigenetic regulation" 精确检索。
PRDM13 is a member of the PRDI-BF1 and RIZ homology (PRDM) family of proteins, which are characterized by a conserved N-terminal PR domain structurally related to SET methyltransferase domains and C-terminal zinc finger motifs. Unlike many PRDM proteins, PRDM13 lacks enzymatic methyltransferase activity due to substitutions in key residues of its PR domain. It functions primarily as a transcriptional regulator, playing critical roles in cell fate specification during embryonic development, particularly in the nervous system. PRDM13 is essential for maintaining neuronal progenitor pools by suppressing premature differentiation. In the developing dorsal spinal cord and brainstem, it inhibits the generation of excitatory glutamatergic neurons while promoting inhibitory GABAergic interneuron formation, ensuring balanced neural circuitry.
Studies highlight its interaction with core neurogenic factors, such as repressing *Neurog1/Neurogenin1* transcription, thereby modulating neuronal subtype specification. Dysregulation of PRDM13 is linked to neurodevelopmental disorders and cancers; loss-of-function mutations are associated with congenital central hypoventilation syndrome and medulloblastoma. Structurally, its zinc fingers mediate DNA binding, while the PR domain facilitates protein interactions, enabling recruitment of chromatin modifiers like histone deacetylases (HDACs) for transcriptional repression.
PRDM13 exemplifies the functional diversity of PRDM proteins, acting as a context-dependent gatekeeper of differentiation pathways. Its roles in neurodevelopment and disease underscore its potential as a therapeutic target for neurological conditions and malignancies. Research continues to explore its regulatory networks and tissue-specific functions beyond the nervous system.
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