| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRCC |
| Uniprot No | Q92733 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-491 aa |
| 活性数据 | MSLVAYASSD ESEPDEAEPE PEEEEAVAPT SGPALGGLFA SLPAPKGPAL LPPPPQMLAP AFPPPLLLPP PTGDPRLQPP PPLPFGLGGF PPPPGVSPAE AAGVGEGLGL GLPSPRGPGL NLPPPIGGAG PPLGLPKPKK RKEPVKIAAP ELHKGDSDSE EDEPTKKKTI LQGSSEGTGL SALLPQPKNL TVKETNRLLL PHAFSRKPSD GSPDTKPSRL ASKTKTSSLA PVVGTTTTTP SPSAIKAAAK SAALQVTKQI TQEEDDSDEE VAPENFFSLP EKAEPPGVEP YPYPIPTVPE ELPPGTEPEP AFQDDAANAP LEFKMAAGSS GAPWMPKPGD DYSYNQFSTY GDANAAGAYY QDYYSGGYYP AQDPALVPPQ EIAPDASFID DEAFKRLQGK RNRGREEINF VEIKGDDQLS GAQQWMTKSL TEEKTMKSFS KKKGEQPTGQ QRRKHQITYL IHQAKERELE LKNTWSENKL SRRQTQAKYG F |
| 分子量 | 52.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PRCC蛋白的示例参考文献(注：以下内容为模拟文献，仅供参考)：
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1. **文献名称**: *Expression and Functional Analysis of Recombinant PRCC Protein in Renal Cell Carcinoma*
**作者**: Wang, Y. et al.
**摘要**: 研究通过大肠杆菌系统成功表达并纯化了重组人PRCC蛋白，功能实验表明该蛋白在肾癌细胞中通过激活MAPK信号通路促进肿瘤增殖和迁移。
2. **文献名称**: *Structural Characterization of PRCC and Its Role in mRNA Splicing*
**作者**: Lee, S. et al.
**摘要**: 利用重组PRCC蛋白的晶体结构解析，揭示其N端含有一个保守的RNA结合域，体外实验证实PRCC通过调控剪接体复合物的组装影响mRNA前体加工。
3. **文献名称**: *PRCC-TFE3 Fusion Protein Drives Oncogenesis via Transcriptional Dysregulation*
**作者**: Chen, X. et al.
**摘要**: 研究构建了PRCC-TFE3重组融合蛋白，发现其通过异常招募转录共激活因子，导致下游代谢相关基因的过度表达，从而驱动肾癌发生。
4. **文献名称**: *Role of PRCC in Cellular Stress Response Pathways*
**作者**: Gupta, R. et al.
**摘要**: 通过重组PRCC蛋白体外功能实验，发现其与HSP70相互作用并参与细胞应激反应，敲低PRCC会导致内质网应激敏感性增加。
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*注意：上述文献为模拟示例，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实发表的论文。*
Recombinant human PRCC (Proline-Rich Coiled-Coil 2) protein is a engineered version of a naturally occurring protein encoded by the *PRCC* gene, located on chromosome 1q23.1. PRCC is a proline-rich protein containing a conserved coiled-coil domain, facilitating protein-protein interactions. It plays a role in pre-mRNA splicing and transcriptional regulation, particularly in the assembly of spliceosomal complexes. Dysregulation of PRCC is linked to papillary renal cell carcinoma (PRCC), a kidney cancer subtype, where chromosomal translocations t(X;1) fuse *PRCC* with *TFE3*, creating oncogenic fusion proteins that disrupt normal cell cycle control and promote tumorigenesis. Recombinant PRCC is typically produced in *E. coli* or mammalian expression systems, enabling studies on its biochemical properties, structural interactions, and pathological mechanisms. It serves as a critical tool for investigating PRCC-TFE3 fusion-driven oncogenesis, developing diagnostic biomarkers, and screening targeted therapies. Research also explores its potential involvement in other cancers and cellular processes, such as hypoxia response and metabolic signaling. Its recombinant form allows precise manipulation in *in vitro* assays, antibody production, and functional genomics, bridging gaps between genetic findings and therapeutic applications.
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