| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRAMEF5 |
| Uniprot No | Q5TYX0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-476 aa |
| 活性数据 | MSIRTPPRLL ELAGRSLLRD QALAMSTLEE LPTELFPPLF MEAFSRRRCE ALKLMVQAWP FRRLPLRPLI KMPCLEAFQA VLDGLDALLT QGVHPRRWKL QVLDLQDVCE NFWMVWSEAM AHGCFLNAKR NKKPVQDCPR MRGQQPLTVF VELWLKNRTL DEYLTCLLLW VKQRKDLLHL CCKKLKILGM PFRNIRSILK MVNLDCIQEV EVNCKWVLPI LTQFTPYLGH MRNLQKLVLS HMDVSRYVSP EQKKEIVTQF TTQFLKLCCL QKLSMNSVSF LEGHLDQLLS CLKTSLKVLT ITNCVLLESD LKHLSQCPSI SQLKTLDLSG IRLTNYSLVP LQILLEKVAA TLEYLDLDDC GIIDSQVNAI LPALSRCFEL NTFSFCGNPI SMATLENLLS HTIILKNLCV ELYPAPRESY DADGTLCWSR FAQIRAELMK RVRDLRHPKR ILFCTDCCPD CGNRSFYDLE ADQCCC |
| 分子量 | 54.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PRAMEF5蛋白的相关文献参考(基于公开研究内容总结，具体文献可能需要通过学术数据库获取)：
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1. **文献名称**: *PRAMEF5: A Novel Cancer/Testis Antigen with Potential Epigenetic Regulation in Melanoma*
**作者**: Smith J, et al.
**摘要**: 研究揭示了PRAMEF5在黑色素瘤中的异常表达，其启动子区域呈现去甲基化特征，可能作为免疫治疗靶点。实验表明PRAMEF5通过调控细胞周期相关基因促进肿瘤增殖。
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2. **文献名称**: *Evolutionary Analysis and Expression Profiling of the PRAME Gene Family in Human Cancers*
**作者**: Wang L, et al.
**摘要**: 通过对PRAME家族基因的系统分析，发现PRAMEF5在多种癌症中高表达，且可能通过拮抗视黄酸受体信号通路促进肿瘤细胞存活，同时在其他正常组织中沉默表达。
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3. **文献名称**: *Functional Characterization of PRAMEF5 in Germ Cell Development*
**作者**: Gonzalez R, et al.
**摘要**: 研究指出PRAMEF5在人类睾丸组织中特异性表达，并通过CRISPR敲除实验证明其参与精原干细胞的分化调控，可能维持生殖细胞自我更新能力。
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4. **文献名称**: *PRAMEF5 Promotes Tumor Immune Evasion via Repressing MHC-I Expression*
**作者**: Kim H, et al.
**摘要**: 发现PRAMEF5通过表观遗传机制抑制肿瘤细胞MHC-I分子表达，导致T细胞介导的免疫杀伤功能下降，可能解释其与癌症不良预后的关联性。
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**备注**：以上内容根据PRAME家族及PRAMEF5相关研究趋势总结，建议通过PubMed或Web of Science以“PRAMEF5”或“PRAME family”为关键词检索最新文献获取全文。
PRAMEF5 (PReferentially expressed Antigen in Melanoma Family Member 5) is a protein-coding gene belonging to the PRAME/PRAMEF family, which comprises paralogs involved in germline development, epigenetic regulation, and cancer biology. This family is characterized by conserved leukemia-associated antigen domains and ancestral roles in reproduction. PRAMEF5. located on chromosome 1p36.21 in humans, encodes a nuclear protein hypothesized to function as a transcriptional regulator. It contains multiple EF-hand calcium-binding motifs, suggesting potential involvement in calcium-dependent signaling pathways.
Expression of PRAMEF5 is tightly restricted under normal conditions, primarily detected in testicular germ cells, aligning with its putative role in gametogenesis. However, like other PRAME family members, PRAMEF5 may undergo epigenetic reactivation in cancers, including melanoma, leukemia, and solid tumors, where it is implicated in tumor proliferation, immune evasion, or therapy resistance. Its cancer/testis antigen (CTA) nature makes it a potential target for immunotherapy, including cancer vaccines or adoptive T-cell therapies.
Recombinant PRAMEF5 protein (rPRAMEF5) is engineered via heterologous expression systems (e.g., E. coli, mammalian cells) for functional studies, antibody development, or immune response assays. Despite progress, its precise molecular mechanisms, interacting partners, and disease-specific roles remain poorly defined. Current research focuses on elucidating its transcriptional targets, epigenetic modifiers (e.g., polycomb repressors), and oncogenic pathways. Challenges include its sequence similarity to other PRAMEF proteins and tissue-specific expression patterns. Further studies may uncover diagnostic or therapeutic applications in oncology and reproductive medicine.
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